SerpinA8 / Angiotensinogen / AGT Antibody (Antigen Affinity Purified)

Serpin A8 / Angiotensinogen / AGT Antibody ( Antigen Affinity Purified )

Serpin A8 / Angiotensinogen / AGT Antibody Product Information

• Immunogen: Recombinant human SerpinA8 protein ( Catalog#10994-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human SerpinA8 / angiotensinogen ( rh SerpinA8 ; Catalog#10994-H08H ; NP_000020.1 ; Met 1-Ala 485 ). SerpinA8 specific IgG was purified by human Serpin A8 affinity chromatography.

Serpin A8 / Angiotensinogen / AGT Antibody Usage Guide

• Specificity: Human Serpin A8 / Angiotensinogen
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human AGT in WB. Using a DAB detection system, the detection limit for human AGT is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.5 -1.0 μg/mL with the appropriate secondary reagents to detect human SerpinA8. The detection limit for human SerpinA8 is 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Serpin A8 / Angiotensinogen / AGT Antibody Background

Angiotensinogen, also known as AGT and SERPINA8, is a member of the serpin family. It is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. It is expressed by the liver and secreted in plasma. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD).

References

1. Goodfriend, TL. et al.,1975, Circ. Res. 36: 38-48.
2. Weir, MR. et al., 1999, Am. J. Hypertens. 12: 205-13.
3. Rice, GI. et al., 2004, Biochem. J. 383: 45-51.
4. Jankowski, V. et al., 2007, Arterioscler Thromb Vasc Biol. 27(2): 297-302.