AGT / SerpinA8 Protein (His Tag)

Angiotensinogen ( AGT / SerpinA8 ) Protein Datasheet

AGT / SerpinA8 Protein Product Information

• Synonym: ANHU, FLJ92595, FLJ97926, SERPINA8
• Protein Construction: A DNA sequence encoding the human angiotensinogen (NP_000020.1) ( Met 1-Ala 485 ) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

AGT / SerpinA8 Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 34
• Molecular Mass: The recombinant human angiotensinogen comprises 463 amino acids and has a calculated molecular mass of 51.2 kDa. As a result of glycosylation, the apparent molecular mass of rh angiotensinogen is approximately 55-60 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE:

AGT / SerpinA8 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

AGT / SerpinA8 Protein Description

Angiotensinogen, also known as AGT and SERPINA8, is a member of the serpin family. It is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. It is expressed by the liver and secreted in plasma. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD).

References

1. Goodfriend, TL. et al.,1975, Circ. Res. 36: 38-48.
2. Weir, MR. et al., 1999, Am. J. Hypertens. 12: 205-13.
3. Rice, GI. et al., 2004, Biochem. J. 383: 45-51.
4. Jankowski, V. et al., 2007, Arterioscler Thromb Vasc Biol. 27(2): 297-302.