|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
ACY1 was immunoprecipitated using:
Lane A:0.5 mg k562 Whole Cell Lysate2 µL anti-ACY1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-ACY1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 46 kDa
Observed band size: 40 kDa
Anti-ACY1 rabbit polyclonal antibody at 1:500 dilution
Lane A: K562 Whole Cell LysateLysates/proteins at 60 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:46 kDa
Observed band size:40 kDa
Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Aminoacylase 1 Antibody||Rabbit||Polyclonal||ELISA||50008-RP01|
|Anti-Aminoacylase 1 Antibody||Rabbit||Polyclonal||WB,ELISA,IHC-P,IP||50008-T60|
|Anti-Aminoacylase 1 Antibody||Rabbit||Monoclonal||FCM,ICC/IF,IF||10549-R006|
|Anti-Aminoacylase 1 Antibody (FITC)||Rabbit||Monoclonal||FCM||10549-R006-F|
|Anti-Aminoacylase 1 Antibody||Rabbit||Polyclonal||ELISA||10549-RP01|
|Anti-Aminoacylase 1 Antibody||Mouse||Monoclonal||WB,ELISA||10549-MM01|
|Anti-Aminoacylase 1 Antibody||Mouse||Monoclonal||IHC-P||10549-MM02|
|Anti-Aminoacylase 1 Antibody||Rabbit||Monoclonal||IHC-P||10549-R128|