Anti-Aminoacylase 1 Antibody

Cat: 10549-RP02
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Anti-Aminoacylase 1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Aminoacylase 1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Aminoacylase 1
Immunogen
Recombinant Human ACY1 protein (Catalog#10549-H08B)
Preparation
Produced in rabbits immunized with purified, recombinant Human ACY1 (rh ACY1; Catalog#10549-H08B; NP_000657.1; Met 1-Ser 408). ACY1 specific IgG was purified by human ACY1 affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Aminoacylase 1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Aminoacylase 1 Antibody (Rabbit Polyclonal antibody) Images
Human Aminoacylase-1/ACY1 Immunoprecipitation(IP) 8001

ACY1 was immunoprecipitated using:

Lane A:0.5 mg k562 Whole Cell Lysate

2 µL anti-ACY1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ACY1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 46 kDa

Observed band size: 40 kDa

Human Aminoacylase-1/ACY1 Western blot (WB) 13247

Anti-ACY1 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lysates/proteins at 60 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:46 kDa

Observed band size:40 kDa

Anti-Aminoacylase 1 Antibody Alternative Names
Anti-ACY-1 Antibody;Anti-ACY1D Antibody;Anti-HEL-S-5 Antibody
Aminoacylase 1 Background Information

Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.

Full Name
aminoacylase 1
References
  • Sommer A, et al. (2011) The molecular basis of aminoacylase 1 deficiency. Biochim Biophys Acta. 1812(6): 685-90.
  • Maceyka M, et al. (2004) Aminoacylase 1 is a sphingosine kinase 1-interacting protein. FEBS Lett. 568(1-3): 30-4.
  • Cook RM, et al.(1993) Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer. J Biol Chem. 268(23): 17010-7.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Aminoacylase 1 Antibody Rabbit Polyclonal ELISA 50008-RP01
    Anti-Aminoacylase 1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50008-T60
    Anti-Aminoacylase 1 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 10549-R006
    Anti-Aminoacylase 1 Antibody (FITC) Rabbit Monoclonal FCM 10549-R006-F
    Anti-Aminoacylase 1 Antibody Rabbit Polyclonal ELISA 10549-RP01
    Anti-Aminoacylase 1 Antibody Mouse Monoclonal WB,ELISA 10549-MM01
    Anti-Aminoacylase 1 Antibody Mouse Monoclonal IHC-P 10549-MM02
    Anti-Aminoacylase 1 Antibody Rabbit Monoclonal IHC-P 10549-R128
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