Anti-Aminoacylase 1 Antibody

Cat: 10549-MM01
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Anti-Aminoacylase 1 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-Aminoacylase 1 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Aminoacylase 1
No cross-reactivity in ELISA with Insect cell lysate
Recombinant Human ACY1 protein (Catalog#10549-H08B)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACY1 (rh ACY1; Catalog#10549-H08B; NP_000657.1; Met 1-Ser 408). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #7A11C6C2
Protein A
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Aminoacylase 1 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Aminoacylase 1 Antibody (Mouse Monoclonal antibody) Images
Human Aminoacylase-1/ACY1 Western blot (WB) 13246

Anti-ACY1 mouse monoclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:45 kDa

Anti-Aminoacylase 1 Antibody Alternative Names
Anti-ACY-1 Antibody;Anti-ACY1D Antibody;Anti-HEL-S-5 Antibody
Aminoacylase 1 Background Information

Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.

Full Name
aminoacylase 1
  • Sommer A, et al. (2011) The molecular basis of aminoacylase 1 deficiency. Biochim Biophys Acta. 1812(6): 685-90.
  • Maceyka M, et al. (2004) Aminoacylase 1 is a sphingosine kinase 1-interacting protein. FEBS Lett. 568(1-3): 30-4.
  • Cook RM, et al.(1993) Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer. J Biol Chem. 268(23): 17010-7.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Aminoacylase 1 Antibody Rabbit Polyclonal ELISA 50008-RP01
    Anti-Aminoacylase 1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50008-T60
    Anti-Aminoacylase 1 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 10549-R006
    Anti-Aminoacylase 1 Antibody (FITC) Rabbit Monoclonal FCM 10549-R006-F
    Anti-Aminoacylase 1 Antibody Rabbit Polyclonal ELISA 10549-RP01
    Anti-Aminoacylase 1 Antibody Mouse Monoclonal IHC-P 10549-MM02
    Anti-Aminoacylase 1 Antibody Rabbit Polyclonal WB,ELISA,IP 10549-RP02
    Anti-Aminoacylase 1 Antibody Rabbit Monoclonal IHC-P 10549-R128
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