Alpha-2-glycoprotein / AZGP1 Protein (His Tag)

Alpha-2-glycoprotein 1, zinc-binding Protein Datasheet

Alpha-2-glycoprotein / AZGP1 Protein Product Information

• Synonym: AZGP1, ZAG, ZNGP1
• Protein Construction: A DNA sequence encoding the human AZGP1 (P25311) (Met1-Ser298) with a C-terminal polyhistidine tag was expressed.
• Source: Human
• Expression Host: Human Cells

Alpha-2-glycoprotein / AZGP1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 21
• Molecular Mass: The recombinant human AZGP1 comprises 289 amino acids and has a predicted molecular mass of 33.6 kDa. The apparent molecular mass of the protein is approximately 41 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Alpha-2-glycoprotein / AZGP1 protein

Alpha-2-glycoprotein / AZGP1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Alpha-2-glycoprotein / AZGP1 Protein Description

Alpha-2-glycoprotein, also known as AZGP1, is a member of the MHC class I family. It has been identified as a novel adipokine since it is produced by both white and brown fat adipocytes and may act in a local autocrine fashion in the reduction of adiposity in cachexia. Alpha-2-glycoprotein can be detected in body fluids such as serum, sweat, and seminal and breast cyst fluids. It has been shown that alpha-2-glycoprotein can stimulate lipolysis by adipocytes in vivo and in vitro. Thus it is believed that alpha-2-glycoprotein plays an important role in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and it also regulates melanin production by normal and malignant melanocytes. Alpha-2-glycoprotein may bind polyunsaturated fatty acids. It can cause the extensive fat loss associated with some advanced cancer.

References

1. Tada T. et al., 1991, J Histochem Cytochem. 39 (9): 1221-6.
2. Vanni H. et al., 2009, Chest. 135 (5): 1197-208.
3. Ueyama H. et al., 1991, Biochem Biophys Res Commun. 177 (2): 696-703.