Aequorea victoria GFP Protein (His Tag)

Green fluorescence protein Protein Datasheet

GFP Protein Product Information

• Synonym: GFP
• Protein Construction: A DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser 2-Lys 238) was expressed, with a polyhistide tag at the N-terminus.
• Source: Aequorea victoria
• Expression Host: E. Coli

GFP Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant Aequorea victoria GFP consisting of 253 amino acids and has a calculated molecular mass of 28.7KDa. It migrates as an 35 kDa band in SDS-PAGE under reducing conditions as predicted.
• Formulation: Lyophilized from sterile PBS, pH 7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GFP protein

GFP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GFP Protein Description

The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65–Tyr66–Gly67 that lead to chromophore formation. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has always been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.

References

1. Nagai, M. et al., 1985, Gene. 36:183-188.
2. Jacobs, P. et al., 1985, DNA. 4:139-146.
3. Geiger, M. et al., 1989, Blood. 74 (2): 722-728.
4. Ploug, M. et al., 2002, Biochem. Soc. Trans. 30 (2): 177–183.
5. Duffy, M.J., 2002, Biochem. Soc. Trans. 30: 207-210.
6. Alfano, M. et al., 2004, J. Leukoc. Biol. 74 (5): 750–756.