Adiponectin / Acrp30 / ADIPOQ Protein (Cytokine)

Adiponectin Protein Datasheet

ADIPOQ/ ACDC Protein Product Information

• Synonym: ADIPOQ, ACDC, ACRP30, APM1, GBP28
• Protein Construction: A DNA sequence encoding the human ADIPOQ (Q15848) (Met1-Asn244) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Human
• Expression Host: Human Cells

ADIPOQ/ ACDC Protein QC Testing

• Purity: >97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 19
• Molecular Mass: The recombinant human ADIPOQ /Fc is a disulfide-linked homodimer. The reduced monomer comprises 467 amino acids and has a predicted molecular mass of 51.6 kDa. The apparent molecular mass of the protein is approximately 55-60 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ADIPOQ/ ACDC protein

ADIPOQ/ ACDC Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ADIPOQ/ ACDC Protein Description

Adiponectin (ADIPOQ), also known as Adipocyte, C1q and collagen domain-containing protein, 30 kDa adipocyte complement-related protein, adipocyte complement-related 30 kDa protein, adipose most abundant gene transcript 1 protein, gelatin-binding protein, ADIPOQ and ACDC, is a secreted protein which contains oneC1q domain and onecollagen-like domain. Adiponectin/Acrp30/ADIPOQ is an important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Adiponectin/Acrp30/ADIPOQ stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Adiponectin/Acrp30/ADIPOQ antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Adiponectin/Acrp30/ADIPOQ inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. It may play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

References

1. Maeda K. et al., 1996, Biochem Biophys Res Commun. 221: 286-9.
2. Saito K. et al., 1999, Gene. 229: 67-73.
3. Yamauchi T. et al., 2001, Nat Med. 7: 941-6.
4. Zhang Z. et al., 2004, Protein Sci. 13: 2819-24.
5. Takahashi M. et al., 2000, Int J Obes Relat Metab Disord. 24: 861-8.