Human APC partial Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG11274-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

APC cDNA Clone Datasheet

APC cDNA Clone Product Information

Gene Bank Ref.ID:	NM_000038.4
cDNA Size:	1110
Synonym:	GS; DP2; DP3; BTPS2; DP2.5; APC
Source:	Human

APC cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens adenomatous polyposis coli DNA.
Vector:	Cloning Vector: pMD18-T Simple Vector. Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence.

APC cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

APC cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

APC cDNA Clone Sequence and Quality Control

Sequence:

1        ACTAGGTCCC AGGCACAAAC TCCAGTTTTA AGTCCTTCCC TTCCTGATAT GTCTCTATCC
61       ACACATTCGT CTGTTCAGGC TGGTGGATGG CGAAAACTCC CACCTAATCT CAGTCCCACT
121      ATAGAGTATA ATGATGGAAG ACCAGCAAAG CGCCATGATA TTGCACGGTC TCATTCTGAA
181      AGTCCTTCTA GACTTCCAAT CAATAGGTCA GGAACCTGGA AACGTGAGCA CAGCAAACAT
241      TCATCATCCC TTCCTCGAGT AAGCACTTGG AGAAGAACTG GAAGTTCATC TTCAATTCTT
301      TCTGCTTCAT CAGAATCCAG TGAAAAAGCA AAAAGTGAGG ATGAAAAACA TGTGAACTCT
361      ATTTCAGGAA CCAAACAAAG TAAAGAAAAC CAAGTATCCG CAAAAGGAAC ATGGAGAAAA
421      ATAAAAGAAA ATGAATTTTC TCCCACAAAT AGTACTTCTC AGACCGTTTC CTCAGGTGCT
481      ACAAATGGTG CTGAATCAAA GACTCTAATT TATCAAATGG CACCTGCTGT TTCTAAAACA
541      GAGGATGTTT GGGTGAGAAT TGAGGACTGT CCCATTAACA ATCCTAGATC TGGAAGATCT
601      CCCACAGGTA ATACTCCCCC GGTGATTGAC AGTGTTTCAG AAAAGGCAAA TCCAAACATT
661      AAAGATTCAA AAGATAATCA GGCAAAACAA AATGTGGGTA ATGGCAGTGT TCCCATGCGT
721      ACCGTGGGTT TGGAAAATCG CCTGAACTCC TTTATTCAGG TGGATGCCCC TGACCAAAAA
781      GGAACTGAGA TAAAACCAGG ACAAAATAAT CCTGTCCCTG TATCAGAGAC TAATGAAAGT
841      TCTATAGTGG AACGTACCCC ATTCAGTTCT AGCAGCTCAA GCAAACACAG TTCACCTAGT
901      GGGACTGTTG CTGCCAGAGT GACTCCTTTT AATTACAACC CAAGCCCTAG GAAAAGCAGC
961      GCAGATAGCA CTTCAGCTCG GCCATCTCAG ATCCCAACTC CAGTGAATAA CAACACAAAG
1021     AAGCGAGATT CCAAAACTGA CAGCACAGAA TCCAGTGGAA CCCAAAGTCC TAAGCGCCAT
1081     TCTGGGTCTT ACCTTGTGAC ATCTGTTTAA

Quality Control: cDNA sequencing results is available upon request.