Anti-ACTA2 Antibody

Anti-ACTA2 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-ACTA2 Antibody
Validated applications
WB,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human ACTA2
Immunogen
A synthetic peptide corresponding to the N-terminus of the human Alpha-Smooth Muscle Actin (ACTA2).
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the N-terminus of the human Alpha-Smooth Muscle Actin (ACTA2), and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ACTA2 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:2000-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ACTA2 Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of alpha smooth muscle actin in human small intestine with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).

Anti-ACTA2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lane D: HEK293 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:42 kDa

Observed band size:42 kDa

Immunochemical staining of alpha smooth muscle actin in mouse small intestine with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Immunochemical staining of alpha smooth muscle actin in rat small intestine with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Immunochemical staining of alpha smooth muscle actin in human heart with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of alpha smooth muscle actin in human skeletal muscle with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of alpha smooth muscle actin in human breast (from 3 donors) with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of alpha smooth muscle actin in human breast carcinoma (from 3 donors) with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of alpha smooth muscle actin in mouse uterus with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of alpha smooth muscle actin in mouse heart with rabbit polyclonal antibody at 1:40000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of alpha smooth muscle actin in mouse skeletal muscle with rabbit polyclonal antibody at 1:40000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of alpha smooth muscle actin in rat uterus with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of alpha smooth muscle actin in rat heart with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of alpha smooth muscle actin in rat skeletal muscle with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.

Anti-ACTA2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: NIH3T3 Whole Cell Lysate

Lane C: Mouse heart tissue lysate

Lane D: Mouse slpeen tissue lysate

Lane E: Mouse lung tissue lysate

Lane F: Mouse brain tissue lysate

Lane G: Rat heart tissue lysate

Lane H: Rat liver tissue lysate

Lane I: Rat lung tissue lysate

Lane J: Rat kidney tissue lysate

Lane K: Rat brain tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:44 kDa

Observed band size:44 kDa

Anti-ACTA2 Antibody: Alternative Names

Anti-AAT6 Antibody; Anti-ACTSA Antibody; Anti-MYMY5 Antibody

ACTA2 Background Information

Actins are globular multi-functional proteins which can be detected in all eukaryotic cells. In vertebrates, there are three main groups of actins that possess slightly different functions: alpha, beta, and gamma. The alpha actins, found in muscle tissues, are a major constituent of the contractile apparatus. Beta-actin, found at the expanding edge of cells, uses the projection of its cellular structure as its mean of mobility. Gamma-actin is found in the filaments of stress fibres. ACTA2 is an alpha actin that is found in skeletal muscle. Expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.
Full Name
actin, alpha 2, smooth muscle, aorta
References
  • Ueyama H, et al., 1990, Jinrui Idengaku Zasshi. 35(2): 145-50.
  • Snásel J, et al., 1997, Folia Biol. 42(5): 227-30.
  • Adams LD, et al., 1992, AIDS Res Hum. 8(2): 291-5.
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