AACS / ACSF1 Protein

Acyl-CoA Synthetase Family Member 1 ( AACS / ACSF1 ) Protein

AACS / ACSF1 Protein Product Information

• Synonym: ACSF1, FLJ12389, FLJ41251, SUR-5, AACS
• Protein Construction: A DNA sequence encoding the full length of human AACS isoform a ( NP_076417.2 ) ( Met 1 - Phe 672 ) was expressed, with a polyhistidine tag at the N-terminus
• Source: Human
• Expression Host: Baculovirus-Insect cells

AACS / ACSF1 Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human AACS consists of 690 amino acids and predicts a molecular mass of 77 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 20mM Tris, 500 mM NaCl, pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: AACS protein

AACS / ACSF1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

AACS / ACSF1 Protein Description

Acetoacetyl-CoA synthetase, also known as Acyl-CoA synthetase family member 1, Protein sur-5 homolog, AACS, and ACSF1, is a member of the ATP-dependent AMP-binding enzyme family. AACS is highly expressed in kidney, heart and brain, but low in liver. AACS is an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies. AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. AACS was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity. Moreover, AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. AACS is a novel cytosolic ketone body (acetoacetate)-specific ligase, the physiological role of which remains to be elucidated.

References

1. Yamasaki, M. 2005, Biochem Biophys Res Commun. 335 (1): 215-9.
2. Hasegawa, S. et al., 2008, Biochim Biophys Acta. 1779 (6-7): 414-9.
3. Aguiló, F. et al., 2010, Biochem J. 427 (2): 255-64.