AACS / ACSF1 Antibody

Acetoacetyl-CoA Synthetase ( AACS / ACSF1 ) Antibody

AACS / ACSF1 Antibody Product Information

• Immunogen: Recombinant human AACS protein ( Catalog#11117-H07B )
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 7H6F3E1
• Ig Type: Mouse IgG2b
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, insect cell-derived, recombinant human AACS ( rh AACS ; Catalog#11117-H07B ; NP_076417.2 ; Met 1 - Phe 672 ).The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography

AACS / ACSF1 Antibody Usage Guide

• Specificity: Human AACS / ACSF1
• No cross-reactivity with Baculovirus lysate in ELISA
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human AACS in WB. Using a DAB detection system, the detection limit for human AACS is approximately 4 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human AACS. The detection limit for human AACS is 0.039 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

AACS / ACSF1 Antibody Background

Acetoacetyl-CoA synthetase, also known as Acyl-CoA synthetase family member 1, Protein sur-5 homolog, AACS, and ACSF1, is a member of the ATP-dependent AMP-binding enzyme family. AACS is highly expressed in kidney, heart and brain, but low in liver. AACS is an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies. AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. AACS was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity. Moreover, AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. AACS is a novel cytosolic ketone body (acetoacetate)-specific ligase, the physiological role of which remains to be elucidated

References

1. Yamasaki, M. 2005, Biochem Biophys Res Commun. 335 (1): 215-9.
2. Hasegawa, S. et al., 2008, Biochim Biophys Acta. 1779 (6-7): 414-9.
3. Aguiló, F. et al., 2010, Biochem J. 427 (2): 255-64.