Anti-ATP1B4 Antibody

Cat: 13027-R108
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Anti-ATP1B4 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-ATP1B4 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human ATP1B4
Immunogen
Recombinant Human ATP1B4 protein
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ATP1B4 (mature form, NP_036201.1; Tyr 132-Thr 357).
Source
Monoclonal Rabbit IgG Clone #108
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ATP1B4 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ATP1B4 Antibody (Rabbit Monoclonal antibody) Images
Mouse MAP2 Immunoprecipitation(IP) 8238

ATP1B4 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

0.5 µL anti-ATP1B4 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ATP1B4 rabbit monoclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 40 kDa

Observed band size: 40 kDa

Human ATP1B4 Western blot (WB) 13519

Anti-ATP1B4 rabbit monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:42 kDa

Observed band size:38 kDa

ATP1B4 Background Information

ATP1B4 is a member of the X(+)/potassium ATPases subunit beta family. It is highly expressed in skeletal muscle and at a lower level in heart. ATP1B4 gene can be found in all vertebrate genomes sequenced to date. However, this gene has undergone a change in function in placental mammals compared to other species. Specifically, in fish, avian, and amphibian species, this gene encodes plasma membrane-bound beta-subunits of Na, K-ATPase. In placental mammals, the encoded protein interacts with the nuclear transcriptional coregulator SKIP and may be involved in the regulation of TGF-beta signaling. ATP1B4 may act as a transcriptional coregulator during muscle development through its interaction with SNW1. Na+, K+-ATPase is an important regulator of intracellular electrolyte levels in most mammalian cells. It is a Mg2+-dependent transport pump responsible for maintaining the low intracellular Na+:K+ ratio that is essential for cell homeostasis and physiological function. It catalyzes the active uptake of K+ and extrusion of Na+ at the expense of hydrolyzing ATP with a stoichiometry of 3 Na+ for 2 K+. ATP1B4 has lost its ancestral function as a Na,K-ATPase beta-subunit.

Full Name
ATPase, Na+/K+ transporting, beta 4 polypeptide
References
  • Pestov NB. et al., 2007, Proc Natl Acad Sci. 104 (27): 11215-20.
  • Kung AW. et al., 2006, Clin Endocrinol. 64 (2): 158-61.
  • Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ATP1B4 Antibody Mouse Monoclonal ELISA 13027-MM04
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