Rat ATP1B3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80384-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ATP1B3 cDNA Clone Datasheet

ATP1B3 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_012913.1
cDNA Size:	840
Gene Synonym:	NKAB3S;Atp1b3
Source:	Rat

ATP1B3 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus ATPase, Na+/K+ transporting, beta 3 polypeptide DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

ATP1B3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ATP1B3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ATP1B3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACGAAGA CTGAGAAGAA ATCCTTCCAC CAGAGCCTGG CCGAGTGGAA GCTGTTCATC
61       TACAACCCGA CCAGCGGAGA GTTTCTGGGG CGCACCTCCA AGAGCTGGGG TCTCATCTTG
121      CTCTTCTACC TAGTTTTTTA TGGGTTCTTG GCTGCACTCT TCACATTCAC CATGTGGGTC
181      ATGCTCCAGA CTCTGAATGA CGAGGTTCCG AAGTACCGAG ACCAGATTCC TAGTCCAGGA
241      CTTATGGTTT TCCCCAAACC ACCGACTGCC TTGGATTATA CATACAGCAT GTCTGACCCA
301      CATACTTATA AAAAGTTTGT TGAAGACCTG AAGAATTTCC TAAAGCCATA TTCTGTGGAA
361      GAACAGAAGA ATCTCACAGA TTGCCCTGGT GGAGCACTTT TTCATCAGGA GGGTCCAGAC
421      TATAGTGCGT GTCAGTTCCC AGTTTCCTTG CTTCAAGAAT GTAGTGGTGT GAATGATTCT
481      AATTTTGGCT ACTCCAAAGG ACAACCTTGT GTTCTTGTGA AGATGAACAG AATAATCGAG
541      TTAGTCCCAG ATGGAGCTCC ATATATTACA TGTATAACAA AGGAAGAAAA CATAGCAAAT
601      ATAGTAACTT ATCCTGATGA TGGACTTATA GACTTAAAGT ATTTTCCATA TTATGGGAAA
661      AAACGGCACG TTGGATATCG ACAGCCCCTA GTTGCCGTCC AGGTCATATT TGGGGCCGAT
721      GCTACCAAGA AAGAAGTAAC AATTGAGTGC CAAATTGATG GAACCAGGAA CCTAAAAAAC
781      AAGAATGAAC GTGACAAGTT CTTGGGACGA GTTTCATTTA AAGTCATAGC ACATGCCTAG

Quality Control:cDNA sequencing results is available upon request.