Mouse ATP1B3 Gene cDNA Clone / ORF Clone

ATP1B3 cDNA Clone Datasheet

ATP1B3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007502.4
• cDNA Size: 837
• Gene Synonym: AA409958; AI664000; AW212096; Atp1b3
• Source: Mouse

ATP1B3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus ATPase, Na+/K+ transporting, beta 3 polypeptide DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

ATP1B3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ATP1B3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ATP1B3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACGAAGA CTGAGAAGAA ATCCTTCCAC CAGAGCCTGG CCGAGTGGAA GCTGTTCATC
61       TACAACCCGA GCAGCGGAGA GTTTCTGGGG CGCACCTCCA AGAGCTGGGG TCTGATCTTG
121      CTCTTCTACC TAGTTTTTTA TGGGTTCTTG GCTGCACTCT TCACATTCAC AATGTGGGCC
181      ATGCTCCAGA CTCTGAATGA TGAAGTTCCG AAATATCGAG ACCAGATTCC TAGTCCAGGA
241      CTTATGGTTT TTCCCAAACC GCAGACTGCC TTGGAATATA CATTCAGCAT GTCTGAGCCA
301      CAGACTTACA AAAAGTTGGT TGAAGACCTG GAGAGTTTCC TAAAGCCATA TTCTGTGGAA
361      GAACAGAAGA ACCTCACAAG TTGTCCTGAT GGAGCGCCTT TTATTCAGCA TGGTCCTGAC
421      TATAGGGCAT GTCAGTTTCC AGTCTCCTTG CTTGAAGAAT GTAGTGGTGT GACTGATGCT
481      AATTTTGGCT ATTCCAAAGG ACAGCCTTGC ATCCTTGTGA AAATGAACAG AATAATCGAT
541      TTAATCCCAG ACGGATATCC ACAAATATCG TGTTTGCCAA AGGAAGAAAA CGCAACTATA
601      GCAACTTATC CTGAATTTGG AGTTTTAGAC TTAAAGTATT TTCCATATTA TGGGAAAAAA
661      CGGCATGTTG GATATCGACA GCCCCTAGTT GCCGTACAGG TCAAATTTGA CTCTGGTCTT
721      AACAAGAAAG AAGTAACAGT TGAGTGCCAT ATTGCTGGAA CCAGGAACCT AAAAAACAAG
781      AATGAGCGTG ACAAGTTCTT GGGACGTGTT TCGTTCAAAG TCACAGCACG AGCCTAG

 

Quality Control:cDNA sequencing results is available upon request.