ATF2 Protein (GST Tag)

Activating transcription factor 2 Protein Datasheet

ATF2 Protein Product Information

• Synonym: CRE-BP1, CREB2, HB16, MGC111558, TREB7
• Protein Construction: A DNA sequence encoding full length of human ATF2 (P15336-1) (Met 1-Ser 505) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

ATF2 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human ATF2/GST chimera consists of 742 amino acids and has a calculated molecular mass of 82.4 KDa. It migrates as an approximately 85KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0, 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ATF2 protein

ATF2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ATF2 Protein Description

Activating transcription factor 2, also known as ATF2, is one member of the leucine zipper family of DNA-binding proteins that binds to the cAMP response element, and its activity is enhanced after phosphorylation by stress-activated protein kinases such as c-Jun N-terminal kinase and p38. Activating transcription factor-2 (ATF2) was found to be a target of the JNK signal transduction pathway. ATF2 was phosphorylated by JNK on two closely spaced threonine residues within the NH2-terminal activation domain. The ATF-2 transcription factor can mediate adenovirus E1A-inducible transcriptional activation. Deletion analysis has indicated that the N-terminal region of ATF-2 is essential for this response. ATF2 is also been reported playing roles in TGF-β signaling. It has been shown that the transcription factor ATF-2 is bound by a hetero-oligomer of Smad3 and Smad4 upon TGF-β stimulation. The binding between ATF-2 and Smad3/4 is mediated via the MH1 region of the Smad proteins and the basic leucine zipper region of ATF-2. These results indicate that ATF-2 plays a central role in TGF-β signaling by acting as a common nuclear target of both Smad and TAK1 pathways.

References

1. Livingstone C. et al., 1995, EMBO J. 14 (8): 1785-97.
2. Gupta S. et al., 1995, Science. 267 (5196): 389-93.
3. Sano YJ. et al., 1999, The Journal of Biological Chemistry. 274: 8949-57.