Human ASPN Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13308-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ASPN cDNA Clone Datasheet

ASPN cDNA Clone Product Information

Gene Bank Ref.ID:	NM_017680.4
cDNA Size:	1140
Gene Synonym:	OS3; PLAP1; PLAP-1; SLRR1C; FLJ20129; ASPN
Source:	Human

ASPN cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens asporin DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

ASPN cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ASPN cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ASPN cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGGAGT ATGTGCTCCT ATTATTCCTG GCTTTGTGCT CTGCCAAACC CTTCTTTAGC
61       CCTTCACACA TCGCACTGAA GAATATGATG CTGAAGGATA TGGAAGACAC AGATGATGAT
121      GATGATGATG ATGATGATGA TGATGATGAT GAGGACAACT CTCTTTTTCC AACAAGAGAG
181      CCAAGAAGCC ATTTTTTTCC ATTTGATCTG TTTCCAATGT GTCCATTTGG ATGTCAGTGC
241      TATTCACGAG TTGTACATTG CTCAGATTTA GGTTTGACCT CAGTCCCAAC CAACATTCCA
301      TTTGATACTC GAATGCTTGA TCTTCAAAAC AATAAAATTA AGGAAATCAA AGAAAATGAT
361      TTTAAAGGAC TCACTTCACT TTATGGTCTG ATCCTGAACA ACAACAAGCT AACGAAGATT
421      CACCCAAAAG CCTTTCTAAC CACAAAGAAG TTGCGAAGGC TGTATCTGTC CCACAATCAA
481      CTAAGTGAAA TACCACTTAA TCTTCCCAAA TCATTAGCAG AACTCAGAAT TCATGAAAAT
541      AAAGTTAAGA AAATACAAAA GGACACATTC AAAGGAATGA ATGCTTTACA CGTTTTGGAA
601      ATGAGTGCAA ACCCTCTTGA TAATAATGGG ATAGAGCCAG GGGCATTTGA AGGGGTGACG
661      GTGTTCCATA TCAGAATTGC AGAAGCAAAA CTGACCTCAG TTCCTAAAGG CTTACCACCA
721      ACTTTATTGG AGCTTCACTT AGATTATAAT AAAATTTCAA CAGTGGAACT TGAGGATTTT
781      AAACGATACA AAGAACTACA AAGGCTGGGC CTAGGAAACA ACAAAATCAC AGATATCGAA
841      AATGGGAGTC TTGCTAACAT ACCACGTGTG AGAGAAATAC ATTTGGAAAA CAATAAACTA
901      AAAAAAATCC CTTCAGGATT ACCAGAGTTG AAATACCTCC AGATAATCTT CCTTCATTCT
961      AATTCAATTG CAAGAGTGGG AGTAAATGAC TTCTGTCCAA CAGTGCCAAA GATGAAGAAA
1021     TCTTTATACA GTGCAATAAG TTTATTCAAC AACCCGGTGA AATACTGGGA AATGCAACCT
1081     GCAACATTTC GTTGTGTTTT GAGCAGAATG AGTGTTCAGC TTGGGAACTT TGGAATGTAA

Quality Control:cDNA sequencing results is available upon request.