Human ASMT Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13887-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ASMT cDNA Clone Datasheet

ASMT cDNA Clone Product Information

Gene Bank Ref.ID:	BC001620
cDNA Size:	897
Gene Synonym:	RP13-297E16.2, ASMTY, HIOMT, HIOMTY,ASMT
Source:	Human

ASMT cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens acetylserotonin O-methyltransferase DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

ASMT cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ASMT cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ASMT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGATCCT CAGAGGACCA GGCCTATCGC CTCCTTAATG ACTACGCCAA CGGCTTCATG
61       GTGTCCCAGG TTCTCTTCGC CGCCTGCGAG CTGGGCGTGT TTGACCTTCT CGCCGAGGCC
121      CCAGGGCCCC TGGACGTGGC GGCAGTGGCT GCAGGTGTGA GGGCCAGCGC CCATGGGACA
181      GAGCTCCTGC TGGACATCTG TGTGTCCCTG AAGCTGCTGA AAGTGGAGAC GAGGGGAGGA
241      AAAGCTTTCT ATCGAAACAC AGAGCTGTCC AGCGACTACC TGACCACGGT CAGCCCGACG
301      TCACAATGCA GCATGCTGAA GTACATGGGC AGGACCAGCT ACCGGTGCTG GGGCCACCTG
361      GCAGACGCCG TGAGAGAAGG AAGGAACCAG TACCTGGAGA CGTTTGGCGT TCCCGCTGAA
421      GAGCTTTTTA CGGCCATCTA CAGGTCCGAG GGCGAGCGGC TACAGTTCAT GCAAGCTCTG
481      CAGGAGGTCT GGAGCGTCAA CGGGAGAAGC GTGCTGACCG CCTTTGACCT GTCAGTGTTC
541      CCACTTATGT GTGACCTTGG TGGGGATTTC TTCAAAGACC CTCTTCCGGA AGCTGATCTG
601      TACATCCTGG CCAGGGTCCT CCATGACTGG GCAGATGGAA AGTGCTCACA CCTGCTGGAG
661      AGGATCTACC ACACTTGCAA GCCAGGTGGT GGCATTCTGG TAATTGAAAG CCTCCTGGAT
721      GAAGACAGGC GAGGTCCTCT GCTCACGCAG CTCTACTCTC TGAACATGCT TGTGCAGACG
781      GAAGGGCAGG AGAGGACCCC CACCCACTAC CACATGCTCC TCTCTTCTGC TGGCTTCAGA
841      GACTTCCAGT TTAAGAAAAC AGGAGCCATT TATGATGCCA TTTTAGCCAG GAAATAA

Quality Control:cDNA sequencing results is available upon request.