ASGR2 Protein (His Tag)

Asialoglycoprotein receptor 2 Protein Datasheet

ASGR2 Protein Product Information

• Synonym: ASGR2, CLEC4H
• Protein Construction: A DNA sequence encoding the human ASGR2 (P07307-1)(Gln80-Ala311) was expressed, with an N-terminal polyhistidine tag.
• Source: Human
• Expression Host: Human Cells

ASGR2 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human ASGR2 comprises 252 amino acids and has a predicted molecular mass of 28.9 kDa. The apparent molecular mass of the protein is approximately 37-42 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ASGR2 protein

ASGR2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ASGR2 Protein Description

ASGR2 is a glycoprotein that forms the asialoglycoprotein receptor, also known as the Ashwell receptor, which is specific for desialylated (galactosyl-terminal) glycoproteins and is expressed exclusively in hepatic parenchymal cells. ASGR2 is a subunit of the asialoglycoprotein receptor. ASGR2 mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The asialoglycoprotein receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. ASGR2 is the less abundant minor subunit.

References

1. Davila S. et al., 2010, Genes Immun. 11 (3): 232-8.
2. Zhang X. et al., 2011, Arch Virol. 156 (4): 637-45.
3. Guy CS. et al., 2011, Hepatology. 54 (3): 1043-50.