Mouse ASGPR1 / ASGR1 Protein

Asialoglycoprotein Receptor 1 ( ASGPR1 / ASGR1 ) Protein

ASGPR1 / ASGR1 Protein Product Information

• Synonym: RP23-172M21.2, ASGPR1, Asgr, Asgr-1
• Protein Construction: A DNA sequence encoding the extracellular domain (Ser 60-Asn 284) of mouse ASGPR1 (NP_033844.1) was expressed with a N-terminal polyhistidine tag
• Source: Mouse
• Expression Host: Human Cells

ASGPR1 / ASGR1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The secreted recombinant mouse ASGR1 consists of 241 amino acids and has a calculated molecular mass of 28 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
• SDS-PAGE: ASGR1 protein

ASGPR1 / ASGR1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ASGPR1 / ASGR1 Protein Description

Mouse asialoglycoprotein receptor (ASGPR) is a heterooligomeric receptor that is abundantly expressed on the sinusoidal surface of the hepatic plasma membrane. It is an endocytic receptor that rapidly binds and internalizes galactose-terminated glycoproteins (asialoglycoproteins or ASGP) from the circulation. The mouse ASGPR belongs to the long-form subfamily of the C-type/Ca²⁺ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type I I transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca²⁺-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The aa sequence of mouse ASGPR1 ECD is 89% and 79% identical to the ASGPR1 ECD of rat and human, respectively.

References

1. Sanford, J. P. et al., 1988, DNA 7:721-728.
2. Takezawa, R. et al., 1993, Biochim. Biophys. Acta 1172 :220-222.
3. Monroe, R. S. et al., 1994, Gene 148: 237-244.
4. Stockert, R. J., 1995, Physiol. Rev. 75(3): 591–609.
5. Braun, J. R. et al., 1996, J Biol Chem. 271 (35): 21160-21166.
6. Meier, M. et al., 2000, J. Mol. Biol. 300: 857-865.
7. Weigel, P. H. et al., 2002, Biochim. Biophys. Acta 1572:341-363.