Anti-Asialoglycoprotein Receptor 1 Antibody

Cat: 50083-RP02
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Anti-Asialoglycoprotein Receptor 1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Asialoglycoprotein Receptor 1 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse Asialoglycoprotein Receptor 1
Immunogen
Recombinant Mouse ASGPR1 / ASGR1 protein (Catalog#50083-M07H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse ASGPR1 / ASGR1 (rM ASGPR1 / ASGR1; Catalog#50083-M07H; NP_033844.1; Ser 60-Asn 284). ASGPR1 / ASGR1 specific IgG was purified by Mouse ASGPR1 / ASGR1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Asialoglycoprotein Receptor 1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Asialoglycoprotein Receptor 1 Antibody (Rabbit Polyclonal antibody) Images
Mouse ASGPR1  Immunohistochemistry(IHC) 3383
Immunochemical staining of mouse ASGR1 in mouse liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Mouse ASGPR1  Immunoprecipitation(IP) 8219

Mouse ASGR1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

0.5 µL anti-Mouse ASGR1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse ASGR1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 42 kDa

Mouse ASGPR1  Western blot (WB) 13642

Anti-ASGR1 rabbit polyclonal antibody at 1:500 dilution

Lane A: NIH3T3 whole cell lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:36 kDa

Anti-Asialoglycoprotein Receptor 1 Antibody Alternative Names
Anti-ASGPR1 Antibody;Anti-Asgr Antibody;Anti-Asgr-1 Antibody;Anti-HL-1 Antibody
Asialoglycoprotein Receptor 1 Background Information

The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.

Full Name
asialoglycoprotein receptor 1
References
  • Yang J, et al. (2006) Antisense oligonucleotides targeted against asialoglycoprotein receptor 1 block human hepatitis B virus replication. J Viral Hepat. 13(3): 158-65.
  • Li Y, et al. (2008) Targeted delivery of macromolecular drugs: asialoglycoprotein receptor (ASGPR) expression by selected hepatoma cell lines used in antiviral drug development. Curr Drug Deliv. 5(4): 299-302.
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