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ASGPR1 / ASGR1 Antibody (Antigen Affinity Purified)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
50083-RP02	100µg ($290)		YES	Bulk Order Inquiring

ASGPR1 / ASGR1 Antibody (Antigen Affinity Purified) Datasheet

Order or Inquire for ASGPR1 / ASGR1 Antibody product	Quality antibodies	Antibody production services
Detection limit is 0.5 ng/lane in WB
Detection limit is 0.00245 ng/well in ELISA

ASGPR1 / ASGR1 Antibody Product Information

Immunogen :	Recombinant Mouse ASGPR1 / ASGR1 ?protein (Catalog#50083-M07H)
Antibody Type :	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type :	Rabbit IgG
Formulation :	0.2 μm filtered solution in PBS with 5% trehalose
Preparation :	Produced in rabbits immunized with purified, human cell-derived, recombinant Mouse ASGPR1 / ASGR1? (rM ASGPR1 / ASGR1 ; Catalog#50083-M07H; NP_033844.1; Ser 60-Asn 284). ASGPR1 / ASGR1? specific IgG was purified by Mouse ASGPR1 / ASGR1 ?affinity chromatography.

ASGPR1 / ASGR1 Antibody Usage Guide

Specificity :	Mouse ASGPR1 / ASGR1
Western blot :	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ASGR1 in WB. Using a DAB detection system, the detection limit for Mouse ASGR1 is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
Direct ELISA :	This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse ASGR1. The detection limit for Mouse ASGR1 is 0.00245 ng/well.
Storage :	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ASGPR1 / ASGR1 Antibody Related Products & Topics

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
ASGPR1/ASGR1	Human	ASGPR1 / ASGR1 Protein,Recombinant	10773-H07H
ASGPR1/ASGR1	Mouse	ASGPR1 / ASGR1 Protein, Recombinant	50083-M07H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Mouse ASGPR1/ASGR1	WB, ELISA	Rabbit Polyclonal Antibody	50083-RP01
Mouse ASGPR1/ASGR1	WB, ELISA	ASGPR1 / ASGR1 Antibody (Antigen Affinity Purified)	50083-RP02

ASGPR1 / ASGR1 Antibody Background

Mouse asialoglycoprotein receptor (ASGPR) is a heterooligomeric receptor that is abundantly expressed on the sinusoidal surface of the hepatic plasma membrane. It is an endocytic receptor that rapidly binds and internalizes galactose-terminated glycoproteins (asialoglycoproteins or ASGP) from the circulation. The mouse ASGPR belongs to the long-form subfamily of the C-type/Ca²⁺ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type I I transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca²⁺-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The aa sequence of mouse ASGPR1 ECD is 89% and 79% identical to the ASGPR1 ECD of rat and human, respectively.

References

Sanford, J. P. et al., 1988, DNA 7:721-728.
Takezawa, R. et al., 1993, Biochim. Biophys. Acta 1172 :220-222.
Monroe, R. S. et al., 1994, Gene 148: 237-244.
Stockert, R. J., 1995, Physiol. Rev. 75(3): 591–609.
Braun, J. R. et al., 1996, J Biol Chem. 271 (35): 21160-21166.
Meier, M. et al., 2000, J. Mol. Biol. 300: 857-865.
Weigel, P. H. et al., 2002, Biochim. Biophys. Acta 1572:341-363.