Rat ASAM Gene cDNA Clone / ORF Clone

ASAM cDNA Clone Datasheet

ASAM cDNA Clone Product Information

• Gene Bank Ref.ID: NM_173154.1
• cDNA Size: 1119
• Gene Synonym: ACAM, CLMP, Ol16, Asam
• Source: Rat

ASAM cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus adipocyte-specific adhesion molecule DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

ASAM cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ASAM cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ASAM cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCCCTCT TCTTCCTCTG GCTAGTAACC TATTATGTTG GAACGCTGGG AACTCACACT
61       GAGATCAAGA GAGTGGCAGA GGAAAAGGTT ACCTTGCCCT GTCACCATCA ATTGGGACTT
121      CCTGAGAAAG ACACCCTGGA CATTGAATGG CTGCTCACGG ATAATGAAGG GAACCAAAAA
181      GTGGTTATTA CGTATTCCAG TCGTCATGTC TACAATAACT TGACTGAGGA GCAGAAGGGC
241      CGAGTGGCCT TTGCCTCCAA CTTCCTGGCG GGAGACGCCT CCCTGCAGAT CGAGCCTCTG
301      AAACCCAGTG ATGAAGGCAG ATACACCTGC AAGGTGAAGA ATTCAGGACG CTATGTCTGG
361      AGCCACGTGA TCTTAAAAGT GCTAGTGAGA CCATCCAAGC CCAAGTGTGA GTTGGAAGGA
421      GAGCCGACGG AAGGAAGTGA CCTGACGCTA CAATGTGAGT CTGCCTCTGG AACCAAGCCC
481      ATTGTGTATT ATTGGCAGCG GATTCGGGAG AAGGAGGGAG AGGATGAACA CCTGCCACCC
541      AAATCTAGAA TCGATTACAA CAACCCTGGC CGAGTGCTGC TGCAGAACCT CACCATGGCC
601      TCCTCTGGGC TCTACCAGTG CACAGCAGGC AACGAGGCTG GAAAGGAGAG CTGCGTGGTA
661      CGAGTGACTG TACAGTATGT GCAGAGCATT GGCATGGTTG CAGGAGCAGT GACAGGCATA
721      GTGGCGGGAG CTCTGCTCAT TTTCCTCTTG ATATGGCTCC TAATACGAAG GAAAAGCAAA
781      GAGAGATATG AGGAAGAAGA CAGACCTAAT GAAATCCGAG AAGACGCTGA AGCGCCCCGC
841      GCCCGCCTTG TGAAACCCAG CTCCTCTTCC TCAGGCTCCC GGAGCTCTCG CTCTGGCTCC
901      TCCTCCACCC GCTCCACCGG GAACAGTGCC TCCAGAAGCC AGCGGACGCT GTCGAGTGAA
961      GCAGCACCAC AGCCCGGGCT AGCCACCCAG GCATACAGCC TAATAGGACC GGAAGTGAGA
1021     GGTTCTGAAC CAAAGAAAGC CCACCATACG ACCCTGACCA AAGCAGAAAC CACACTCAGC
1081     ACAATGCCCA GCCAGAGCAG AGCCTTCCAA ACTGTCTGA

 

Quality Control:cDNA sequencing results is available upon request.