ASAM Antibody

ASAM Antibody Datasheet

ASAM Antibody Product Information

• Immunogen: Recombinant Mouse ASAM / ACAM protein (Catalog#50553-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 001
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse ASAM (rM ASAM; Catalog#50553-M08H; Q8R373-1; Met 1-Met 232).

ASAM Antibody Usage Guide

• Specificity: Mouse ASAM
• No cross-reactivity in ELISA with

  Human ASAM

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse ASAM in WB. Using a DAB detection system, the detection limit for Mouse ASAM is approximately 2 ng/lane under non-reducing conditions.Use of this antibody under reducing conditions is not recommended.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ASAM. The detection limit for Mouse ASAM is approximately 0.0195 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ASAM Antibody Background

Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. The extracellular region of human ASAM contains two potential N-linked glycosylation sites, and two immunoglobulin domains, one variable (V-type) and one constant (C2-type), separated from each other by a J-segment. ASAM is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. Its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity. Human ASAM shares 94% and 93% aa sequence identity with rat and mouse ASAM, respectively.

References

1. Raschperger, E. et al., 2004, J. Biol. Chem. 279: 796-804.
2. Eguchi, J. et al., 2005, Biochem. J. 387: 343-353.