Anti-ASAH2 Antibody

Cat: 50006-R002
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Anti-ASAH2 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-ASAH2 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Mouse
Specificity
Mouse ASAH2
Immunogen
Recombinant Mouse ASAH2 protein (Catalog#50006-M07H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse ASAH2 (rM ASAH2; Catalog#50006-M07H; Thr 34-Thr 756; NP_061300.1).
Source
Monoclonal Rabbit IgG Clone #2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ASAH2 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ASAH2 Antibody Alternative Names
Anti-AI585898 Antibody
ASAH2 Background Information

ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses ‘amucin box’, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.

Full Name
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
References
  • Tani M, et al. (2000) Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. A novel but highly conserved gene family of neutral/alkaline ceramidases. J Biol Chem. 275(15): 11229-34.
  • Franzen R, et al. (2002) Nitric oxide induces neutral ceramidase degradation by the ubiquitin/proteasome complex in renal mesangial cell cultures. FEBS Lett. 532(3): 441-4.
  • Kono M, et al. (2006) Neutral ceramidase encoded by the Asah2 gene is essential for the intestinal degradation of sphingolipids. J Biol Chem. 281(11): 7324-31.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ASAH2 Antibody Rabbit Polyclonal ELISA 50006-RP01
    Anti-ASAH2 Antibody Rabbit Polyclonal ELISA 50006-T16
    Anti-ASAH2 Antibody Rabbit Polyclonal ELISA 50006-RP02
    info info
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