Rat ART4 Gene cDNA Clone / ORF Clone

ART4 cDNA Clone Datasheet

ART4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001173509.1
• cDNA Size: 916
• Gene Synonym: ATR4; Art4
• Source: Rat

ART4 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus ADP-ribosyltransferase 4 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 917-921 deletion mutation.

ART4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ART4 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ART4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGCTGT GGTTTCCAGG AGGGCAGCTC ACTCTGCTGC TGCTGCCGCT GCTGCTGCTG
61       TTGCTCTGGG TCCAGCAGAC ACCGGCGGGG AGCGCTGAGG CTGCTCTTAA GGTCGACGTG
121      GACTTGACAC CGGGTTCCTT CGATGATCAG TACCGAGGCT GTAGCAAACA GGTGCTGGAG
181      GAGCTAAATC AAGGAGACTA TTTCGTGAGG GAGATAGACA CTCATAAGTA TTACTCCAGG
241      GCGTGGCAAA AAGCCCACCT AACCTGGCTG AACCAGGCAA CGTCCCTCCC TGAGAGCATG
301      ACTCCTGTGC ACGCTGTGGC CATCTTGGTT TTCACCTTGA ATCTCAACGT GAGCTCTGAC
361      TTCGCCAAGG CCATGACCAG GGCTTCGGGG TCTCCTGGGC AGTACAGACA GTCATTCCAC
421      TTCAAGTATC TGCACTACTA CCTCACCTCA GCCATCCAGC TGCTGAGGAA AGAGAGCTCT
481      ACCAAGAACG GCAGTCCGTG TTACAGGGTA CACCACAGGA TGAAGGACGT GAACATCGAA
541      GCCAATGTGG GTTCTACCGT TCGATTTGGC CAGTTCCTCT CTGCCTCCCT GCTGAAGGAG
601      GAGACACAGG TGTCTGGAAA CCAAACACTG TTTACCATTT TCACTTGTCT GGGCGCCTCC
661      GTGCAAGACT TCTCTCTCAG GAAGGAAGTC TTGATCCCTC CCTATGAGTT ATTTGAAGTC
721      GTGAGTAAGA ACTGCAGCCT CAAAGGAGAT TTGATAAACT TGCGGTCTGC TGGGAACGTG
781      AGCAGGTACA ACTGCCAGCT GCTGAAAGCT TCCAGCAAGA AGTGCACCCC TGCTCCAATG
841      GTTGTTCTTT GCCTCTGTTT GGTCAGTGTC CTTATCTCGT CCAAAAGCAG AGAGCATAGG
901      AATCTACCGG CTCCCT

 

Quality Control:cDNA sequencing results is available upon request.