All ART4 reagents are produced in house and quality controlled, including 6 ART4 Antibody, 12 ART4 Gene, 5 ART4 Lysate, 5 ART4 Protein, 1 ART4 qPCR. All ART4 reagents are ready to use.
Recombinant ART4 proteins are expressed by HEK293 Cells with fusion tags as C-human IgG1-Fc, C-His.
ART4antibodies are validated with different applications, which are ELISA, WB, IHC-P.
ART4cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each ART4 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, is a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane.