Anti-Arginase Antibody

Cat: 11558-RP02
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Anti-Arginase Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Arginase Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Arginase
Immunogen
Recombinant Human ARG1 / Arginase 1 protein (Catalog#11558-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human ARG1 / Arginase 1 (rh ARG1 / Arginase 1; Catalog#11558-H08H; P05089-1; Met 1-Lys 322). ARG1 / Arginase 1 specific IgG was purified by Human ARG1 / Arginase 1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Arginase Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Arginase Antibody (Rabbit Polyclonal antibody) Images
Human ARG1/Arginase 1 Immunoprecipitation(IP) 8094

ARG1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-ARG1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ARG1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 40 kDa

Observed band size: 40 kDa

Human ARG1/Arginase 1 Western blot (WB) 13416

Anti-ARG1 rabbit polyclonal antibody at 1:500 dilution.

Lane A: Mouse liver tisssue lysate

Lane B: HepG2 Whole Cell Lysate

Lysates/proteins at 30 ug per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:35 kDa

Observed band size:40 kDa

Anti-Arginase Antibody Alternative Names
Anti-ARG1 Antibody
Arginase Background Information

Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.

Full Name
arginase 1
References
  • Durante W, et al. (2007) Arginase: a critical regulator of nitric oxide synthesis and vascular function. Clin Exp Pharmacol Physiol. 34(9): 906-11.
  • Waddington SN. (2002) Arginase in glomerulonephritis. Kidney Int. 61(3): 876-81.
  • Morris SM. (2002). Regulation of enzymes of the urea cycle and arginine metabolism. Annual review of nutrition. 22 (1): 87-105.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Arginase Antibody Rabbit Monoclonal WB,ELISA,IHC-P,IP 11558-R034
    Anti-Arginase Antibody Rabbit Polyclonal ELISA 11558-RP01
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