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Human ARAF Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| HG12209-G |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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ARAF cDNA Clone Datasheet
ARAF cDNA Clone Product Information
| Gene Bank Ref.ID: | BC002466 |
|
cDNA Size: |
1821 |
| Gene Synonym: |
PKS2; A-RAF; ARAF1; RAFA1;ARAF |
| Source: | Human |
ARAF cDNA Clone Description
|
cDNA Description: |
ORF Clone of Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref.ID sequence. |
ARAF cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
ARAF cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
ARAF cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGAGCCAC CACGGGGCCC CCCTGCCAAT GGGGCCGAGC CATCCCGGGC AGTGGGCACC 61 GTCAAAGTAT ACCTGCCCAA CAAGCAACGC ACGGTGGTGA CTGTCCGGGA TGGCATGAGT 121 GTCTACGACT CTCTAGACAA GGCCCTGAAG GTGCGGGGTC TAAATCAGGA CTGCTGTGTG 181 GTCTACCGAC TCATCAAGGG ACGAAAGACG GTCACTGCCT GGGACACAGC CATTGCTCCC 241 CTGGATGGCG AGGAGCTCAT TGTCGAGGTC CTTGAAGATG TCCCGCTGAC CATGCACAAT 301 TTTGTACGGA AGACCTTCTT CAGCCTGGCG TTCTGTGACT TCTGCCTTAA GTTTCTGTTC 361 CATGGCTTCC GTTGCCAAAC CTGTGGCTAC AAGTTCCACC AGCATTGTTC CTCCAAGGTC 421 CCCACAGTCT GTGTTGACAT GAGTACCAAC CGCCAACAGT TCTACCACAG TGTCCAGGAT 481 TTGTCCGGAG GCTCCAGACA GCATGAGGCT CCCTCGAACC GCCCCCTGAA TGAGTTGCTA 541 ACCCCCCAGG GTCCCAGCCC CCGCACCCAG CACTGTGACC CGGAGCACTT CCCCTTCCCT 601 GCCCCAGCCA ATGCCCCCCT ACAGCGCATC CGCTCCACGT CCACTCCCAA CGTCCATATG 661 GTCAGCACCA CGGCCCCCAT GGACTCCAAC CTCATCCAGC TCACTGGCCA GAGTTTCAGC 721 ACTGATGCTG CCGGTAGTAG AGGAGGTAGT GATGGAACCC CCCGGGGGAG CCCCAGCCCA 781 GCCAGCGTGT CCTCGGGGAG GAAGTCCCCA CATTCCAAGT CACCAGCAGA GCAGCGCGAG 841 CGGAAGTCCT TGGCCGATGA CAAGAAGAAA GTGAAGAACC TGGGGTACCG GGACTCAGGC 901 TATTACTGGG AGGTACCACC CAGTGAGGTG CAGCTGCTGA AGAGGATCGG GACGGGCTCG 961 TTTGGCACCG TGTTTCGAGG GCGGTGGCAT GGCGATGTGG CCGTGAAGGT GCTCAAGGTG 1021 TCCCAGCCCA CAGCTGAGCA GGCCCAGGCT TTCAAGAATG AGATGCAGGT GCTCAGGAAG 1081 ACGCGACATG TCAACATCTT GCTGTTTATG GGCTTCATGA CCCGGCCGGG ATTTGCCATC 1141 ATCACACAGT GGTGTGAGGG CTCCAGCCTC TACCATCACC TGCATGTGGC CGACACACGC 1201 TTCGACATGG TCCAGCTCAT CGACGTGGCC CGGCAGACTG CCCAGGGCAT GGACTACCTC 1261 CATGCCAAGA ACATCATCCA CCGAGATCTC AAGTCTAACA ACATCTTCCT ACATGAGGGG 1321 CTCACGGTGA AGATCGGTGA CTTTGGCTTG GCCACAGTGA AGACTCGATG GAGCGGGGCC 1381 CAGCCCTTGG AGCAGCCCTC AGGATCTGTG CTGTGGATGG CAGCTGAGGT GATCCGTATG 1441 CAGGACCCGA ACCCCTACAG CTTCCAGTCA GACGTCTATG CCTACGGGGT TGTGCTCTAC 1501 GAGCTTATGA CTGGCTCACT GCCTTACAGC CACATTGGCT GCCGTGACCA GATTATCTTT 1561 ATGGTGGGCC GTGGCTATCT GTCCCCGGAC CTCAGCAAAA TCTCCAGCAA CTGCCCCAAG 1621 GCCATGCGGC GCCTGCTGTC TGACTGCCTC AAGTTCCAGC GGGAGGAGCG GCCCCTCTTC 1681 CCCCAGATCC TGGCCACAAT TGAGCTGCTG CAACGGTCAC TCCCCAAGAT TGAGCGGAGT 1741 GCCTCGGAAC CCTCCTTGCA CCGCACCCAG GCCGATGAGT TGCCTGCCTG CCTACTCAGC 1801 GCAGCCCGCC TTGTGCCTTA G
Quality Control:cDNA sequencing results is available upon request.
