APOE / apolipoprotein E Protein (Trx & His Tag)

Apolipoprotein E Protein Datasheet

APOE / apolipoprotein E Protein Product Information

• Synonym: AD2, LDLCQ5, LPG, MGC1571
• Protein Construction: A DNA sequence encoding the human APOE (P02649) (Lys 19-His 317) was fused with a Trx and a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: E. Coli

APOE / apolipoprotein E Protein QC Testing

• Purity: > 70 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human APOE/Trx fusion protein consisting of 458 amino acids and has a calculated molecular mass of 51.3 KDa. It migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, 40%glycerol, 0.5mM TCEP. pH 7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: APOE / apolipoprotein E protein

APOE / apolipoprotein E Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

APOE / apolipoprotein E Protein Description

Apolipoprotein E (ApoE) is a 34.2 kDa glycosylated protein with 299 amino acid residues. ApoE is synthesized predominantly in the liver. Hepatic parenchyma cells are the main apoE producing cells in mammalian body, probably accounting for two thirds to three fourths of the plasma apoE. In the nervous system, apoE mRNA is present in neurons, astrocytes, ependymal cells, nonmyelinating Schwann cells. ApoE/Apolipoprotein E produced by mammalian cells exists in different forms, monomers, dimers, modified, unmodified, lipid-rich, and lipid-poor, and so forth. ApoE/Apolipoprotein E plays a double-role in immune responses. Both ApoE/Apolipoprotein E containing lipoproteins and multimers of synthetic apoE peptides inhibited proliferation of cultured lymphocytes by inhibiting DNA synthesis and reducing phospholipid turnover in T cells. ApoE/Apolipoprotein E can also affect innate and acquired immune responses in vitro by its ability to suppress stimulation of cultured neutrophils. ApoE/Apolipoprotein E can bind lipopolysaccharide (LPS), attenuate the inflammatory response, and thus reduce LPS induced lethality. Injection of LPS stimulated higher expression of inflammatory cytokines like interleukin (IL)-1, IL-12, and interferon-γ (IFN-γ), as well as IL-6.

References

1. Mahley RW. 1988, Science. 240 (4852): 622-30.
2. Aleshkov S. et al., 1989, Biochemistry. 36 (34): 10571-80.
3. Hussain MM. et al., 1997, Biochimica et Biophysica Acta. 101 (1): 90-101.