APOE / apolipoprotein E Antibody (Antigen Affinity Purified)

APOE / apolipoprotein E Antibody ( Antigen Affinity Purified ) Datasheet

APOE / apolipoprotein E Antibody Product Information

• Immunogen: Recombinant Human APOE / apolipoprotein E protein (Catalog#10817-H30E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human APOE / apolipoprotein E (rh APOE / apolipoprotein E; Catalog#10817-H30E; P02649; Lys 19-His 317). APOE / apolipoprotein E specific IgG was purified by Human APOE / apolipoprotein E affinity chromatography.

APOE / apolipoprotein E Antibody Usage Guide

• Specificity: Human APOE / apolipoprotein E
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of human APOE in human liver with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections).

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ApoE. The detection limit for Human ApoE is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

APOE / apolipoprotein E Antibody Background

Apolipoprotein E (ApoE) is a 34.2 kDa glycosylated protein with 299 amino acid residues. ApoE is synthesized predominantly in the liver. Hepatic parenchyma cells are the main apoE producing cells in mammalian body, probably accounting for two thirds to three fourths of the plasma apoE. In the nervous system, apoE mRNA is present in neurons, astrocytes, ependymal cells, nonmyelinating Schwann cells. ApoE/Apolipoprotein E produced by mammalian cells exists in different forms, monomers, dimers, modified, unmodified, lipid-rich, and lipid-poor, and so forth. ApoE/Apolipoprotein E plays a double-role in immune responses. Both ApoE/Apolipoprotein E containing lipoproteins and multimers of synthetic apoE peptides inhibited proliferation of cultured lymphocytes by inhibiting DNA synthesis and reducing phospholipid turnover in T cells. ApoE/Apolipoprotein E can also affect innate and acquired immune responses in vitro by its ability to suppress stimulation of cultured neutrophils. ApoE/Apolipoprotein E can bind lipopolysaccharide (LPS), attenuate the inflammatory response, and thus reduce LPS induced lethality. Injection of LPS stimulated higher expression of inflammatory cytokines like interleukin (IL)-1, IL-12, and interferon-γ (IFN-γ), as well as IL-6.

References

1. Mahley RW. 1988, Science. 240 (4852): 622-30.
2. Aleshkov S. et al., 1989, Biochemistry. 36 (34): 10571-80.
3. Hussain MM. et al., 1997, Biochimica et Biophysica Acta. 101 (1): 90-101.