|Datasheet||Specific References||Reviews||Related Products||Protocols|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human APCS Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13610-G-F|
|Human APCS Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13610-G-H|
|Human APCS Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13610-G-M|
|Human APCS Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13610-G-N|
|Human APCS Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13610-G-Y|
Serum amyloid P component (SAP) is the identical serum form of amyloid P component (AP), a highly preserved plasma protein named for its ubiquitous presence in amyloid deposits. As a normal plasma protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". Serum amyloid P component represents another member of the pentraxin family, a highly conserved group of molecules that may play a role in innate immunity. SAP is a key negative regulator for innate immune responses to DNA and may be partly responsible for the insufficient immune responses after DNA vaccinations in humans. SAP suppression may be a novel strategy for improving efficacy of human DNA vaccines and requires further clinical investigations.