APCS / PTX2 / Serum amyloid P component Protein (His Tag)

Amyloid P component, serum Protein Datasheet

APCS / PTX2 / Serum amyloid P component Protein Product Information

• Synonym: APCS, PTX2
• Protein Construction: A DNA sequence encoding the human APCS (P02743) (Met1-Val223) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

APCS / PTX2 / Serum amyloid P component Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His 20
• Molecular Mass: The recombinant human APCS consists of 215 amino acids and predicts a molecular mass of 24.7 KDa. It migrates as an approximately 31 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: APCS / PTX2 / Serum amyloid P component protein

APCS / PTX2 / Serum amyloid P component Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

APCS / PTX2 / Serum amyloid P component Protein Description

Serum amyloid P component (SAP), also known as APCS AND Ptx2, is a 25kDa pentameric protein first identified as the pentagonal constituent of pathological deposits called "amyloid". SAP is a member of the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened β-jellyroll structure similar to that of the legume lectins. SAP has a marked inhibitory effect on the proliferative response of lymphocytes to a variety of T-dependent mitogens. In addition, SAP enhances the formation of active E rosettes, a marker of activated T cells. SAP has certain sequence homology with C-reactive protein (CRP), a classical acute phase response plasma protein, and both of which are evolutionary conserved in all vertebrates and also found in distant invertebrates.

References

1. Cathcart, E. S. et al.,1967, Biochim. Biophy. Acta. 147: 392-3.
2. Levo,Y. et al., 1985, Int Arch Allergy Appl Immunol. 77 (3): 322-5.
3. Emsley, J. et al., 1994, Nature. 367 (6461): 338-45.
4. Pepys, M. B. et al.,1997,Amyloid-International Journal of Experimental and Clinical Investigation. 4: 274-95.