ANXA6 / Annexin A6 / Annexin6 Protein (His Tag)

Annexin A6 Protein Datasheet

ANXA6 / Annexin A6 / Annexin6 Protein Product Information

• Synonym: ANX6, CBP68
• Protein Construction: A DNA sequence encoding the human ANXA6 / Annexin A6 / Annexin6 (P08133) (Met 1-Asp 673) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: E.coli

ANXA6 / Annexin A6 / Annexin6 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human ANXA6 / Annexin A6 / Annexin6 consisting of 683 amino acids and has a calculated molecular mass of 77.2 kDa. It migrates as an approximately 60 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ANXA6 / Annexin A6 / Annexin6 protein

ANXA6 / Annexin A6 / Annexin6 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ANXA6 / Annexin A6 / Annexin6 Protein Description

Annexin A6, also known as ANXA6 or ANXAⅥ, belongs to a family of Ca²⁺-dependent membrane and phospholipid binding proteins. Members of this family have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 6 is phosphorylated in vivo associated with cell growth. Annexin 6 was not phosphorylated in quiescent cells, but was phosphorylated on serine and to a lesser extent threonine, several hours following cell stimulation. Experiment has revealed the presence of annexin A6 on the cell surface of variety cells as putative receptors and / or binding proteins for chondroitin sulfate proteoglycans, helping cells to bind with this extracellular matrix glycosaminoglycan chondroitin sulfate which is related to the cell-substratum adhesion. A post-tranlational modification other than direct protein phosphorylation may influence the activity of annexin6 and provide evidence linking cell growth with regulation of annexin 6 function.

References

1. Takagi H. et al., 2002, J Cell Sci.115 (16): 3309-18.
2. Moss SE. et al., 1992, Biochim Biophys Acta.1160 (1): 120-6.
3. Song G. et al., 1998, J Mol Cell Cardio. 30 (3): 443-51.