Human ANGPT1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13667-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ANGPT1 cDNA Clone Datasheet

ANGPT1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001146.3
cDNA Size:	1497
Gene Synonym:	AGP1; AGPT; ANG1
Source:	Human

ANGPT1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens angiopoietin 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

ANGPT1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ANGPT1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ANGPT1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC
61       AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
121      TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
181      CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
241      CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
301      GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
361      CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
421      CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
481      ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
541      ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
601      ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
661      GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
721      ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
781      CTTGTCAATC TTTGCACTAA AGAAGGTGTT TTACTAAAGG GAGGAAAAAG AGAGGAAGAG
841      AAACCATTTA GAGACTGTGC AGATGTATAT CAAGCTGGTT TTAATAAAAG TGGAATCTAC
901      ACTATTTATA TTAATAATAT GCCAGAACCC AAAAAGGTGT TTTGCAATAT GGATGTCAAT
961      GGGGGAGGTT GGACTGTAAT ACAACATCGT GAAGATGGAA GTCTAGATTT CCAAAGAGGC
1021     TGGAAGGAAT ATAAAATGGG TTTTGGAAAT CCCTCCGGTG AATATTGGCT GGGGAATGAG
1081     TTTATTTTTG CCATTACCAG TCAGAGGCAG TACATGCTAA GAATTGAGTT AATGGACTGG
1141     GAAGGGAACC GAGCCTATTC ACAGTATGAC AGATTCCACA TAGGAAATGA AAAGCAAAAC
1201     TATAGGTTGT ATTTAAAAGG TCACACTGGG ACAGCAGGAA AACAGAGCAG CCTGATCTTA
1261     CACGGTGCTG ATTTCAGCAC TAAAGATGCT GATAATGACA ACTGTATGTG CAAATGTGCC
1321     CTCATGTTAA CAGGAGGATG GTGGTTTGAT GCTTGTGGCC CCTCCAATCT AAATGGAATG
1381     TTCTATACTG CGGGACAAAA CCATGGAAAA CTGAATGGGA TAAAGTGGCA CTACTTCAAA
1441     GGGCCCAGTT ACTCCTTACG TTCCACAACT ATGATGATTC GACCTTTAGA TTTTTGA

Quality Control:cDNA sequencing results is available upon request.