Human AMY2B Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13575-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

AMY2B cDNA Clone Datasheet

AMY2B cDNA Clone Product Information

Gene Bank Ref.ID:	BC011179
cDNA Size:	1536
Gene Synonym:	AMY2;AMY2B
Source:	Human

AMY2B cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens amylase, alpha 2B (pancreatic) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 1510 G/C and 1512 A/T resulting in the amino acid Ala substitution by Pro.

AMY2B cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

AMY2B cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

AMY2B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGTTCT TTCTGTTGCT TTTCACCATT GGGTTCTGCT GGGCTCAGTA TTCCCCAAAT
61       ACACAACAAG GACGGACATC TATTGTTCAT CTGTTTGAAT GGCGATGGGT TGATATTGCT
121      CTTGAATGTG AGCGATATTT AGCTCCCAAG GGATTTGGAG GGGTTCAGGT CTCTCCACCA
181      AATGAAAATG TTGCAATTCA CAACCCTTTC AGACCTTGGT GGGAAAGATA CCAACCAGTT
241      AGCTATAAAT TATGCACAAG ATCTGGAAAT GAAGATGAAT TTAGAAACAT GGTGACTAGA
301      TGTAACAATG TTGGGGTTCG TATTTATGTG GATGCTGTAA TTAATCATAT GTCTGGTAAT
361      GCTGTGAGTG CAGGAACAAG CAGTACCTGT GGAAGTTACT TCAACCCTGG AAGTAGGGAC
421      TTTCCAGCAG TCCCATATTC TGGATGGGAT TTTAATGATG GTAAATGTAA AACTGGAAGT
481      GGAGATATCG AGAACTACAA TGATGCTACT CAGGTCAGAG ATTGTCGTCT GGTTGGTCTT
541      CTTGATCTTG CACTGGAGAA AGATTATGTG CGTTCCAAGA TTGCCGAATA TATGAATCAT
601      CTCATTGACA TTGGTGTTGC AGGGTTCAGA CTTGATGCTT CCAAGCACAT GTGGCCTGGA
661      GACATAAAGG CAATTTTGGA CAAACTGCAT AATCTAAACA GTAACTGGTT CCCTGCAGGA
721      AGTAAACCTT TCATTTACCA GGAGGTAATT GATCTGGGTG GTGAGCCAAT TAAAAGCAGT
781      GACTACTTTG GAAATGGCCG GGTGACAGAA TTCAAGTATG GTGCAAAACT CGGCACAGTT
841      ATTCGCAAGT GGAATGGAGA GAAGATGTCT TACCTAAAGA ACTGGGGAGA AGGTTGGGGT
901      TTCATGCCTT CTGACAGAGC ACTTGTCTTT GTGGATAACC ATGACAATCA ACGAGGACAT
961      GGGGCTGGAG GAGCCTCTAT TCTTACCTTC TGGGATGCTA GGCTGTATAA AATGGCAGTT
1021     GGATTTATGC TTGCTCATCC TTATGGTTTT ACACGAGTAA TGTCAAGCTA CCGTTGGCCA
1081     AGACAGTTTC AAAATGGAAA CGATGTTAAT GATTGGGTTG GGCCACCAAA TAATAATGGA
1141     GTAATTAAAG AAGTTACTAT TAATCCAGAC ACTACTTGTG GCAATGACTG GGTCTGTGAA
1201     CATCGATGGC GCCAAATAAG GAACATGGTT AATTTCCGCA ATGTAGTGGA TGGCCAGCCT
1261     TTTACAAACT GGTATGATAA TGGGAGCAAC CAAGTGGCTT TTGGGAGAGG AAACAGAGGA
1321     TTCATTGTTT TCAACAATGA TGACTGGACA TTTTCTTTAA CTTTGCAAAC TGGTCTTCCT
1381     GCTGGCACAT ACTGTGATGT CATTTCTGGA GATAAAATTA ATGGCAATTG CACAGGCATT
1441     AAAATCTACG TTTCTGACGA TGGCAAAGCT CATTTTTCTA TTAGTAACTC TGCTGAGGAT
1501     CCATTTATTC CTATTCATGC TGAATCTAAA TTATAA

Quality Control:cDNA sequencing results is available upon request.