|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Homo sapiens amylase, alpha 2A (pancreatic) DNA.|
|PA, AMY2, AMY2B, AMY2A|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human AMY2A Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13277-G-F|
|Human AMY2A Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13277-G-H|
|Human AMY2A Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13277-G-M|
|Human AMY2A Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13277-G-N|
|Human AMY2A Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13277-G-Y|
|Product name||Product name|
Alpha-amylase is the major form of amylase found in humans and other mammals. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Alpha-amylase hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Amylases is widely expressed and is most prominent in pancreatic juice and saliva, each of which has its own isoform of human α-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies.