|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of adhesion molecule with Ig-like domain 2 DNA.|
|ALI1, DEGA, AMIGO2|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human AMIGO2 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13735-G-F|
|Human AMIGO2 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13735-G-H|
|Human AMIGO2 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13735-G-M|
|Human AMIGO2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13735-G-N|
|Human AMIGO2 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13735-G-Y|
|Product name||Product name|
AMIGO2 contains Ig-like C2-type (immunoglobulin-like) domain, 6 LRR (leucine-rich) repeats, 1 LRRCT domain and 1 LRRNT domain. It belongs to the immunoglobulin superfamily, AMIGO family. AMIGO2 may mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. It is required for depolarization-dependent survival of cultured cerebellar granule neurons. AMIGO2 may also contribute to signal transduction through its intracellular domain. It may play a role in the tumorigenesis of a subset of gastric adenocarcinomas. AMIGO2 is highly expressed in breast, ovary, cervix, and uterus.