Human ALPL / TNAP Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10440-C	Vector 3.1 ($295) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ALPL / TNAP cDNA Clone Datasheet

ALPL / TNAP cDNA Clone Product Information

Gene Bank Ref.ID:	NM_000478.4
cDNA Size:	1575
Gene Synonym:	HOPS; TNAP; TNSALP; AP-TNAP; FLJ40094; FLJ93059; MGC161443; MGC167935; ALPL
Source:	Human

ALPL / TNAP cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens alkaline phosphatase, liver/bone/kidney DNA.
Vector:	Vector 3.1. Restriction site: KpnI + XhoI Optional Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

ALPL / TNAP cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ALPL / TNAP cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ALPL / TNAP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA
61       GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
121      CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
181      GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
241      CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
301      TACAACACCA ATGCCCAGGT CCCTGACAGC GCCGGCACCG CCACCGCCTA CCTGTGTGGG
361      GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
421      ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
481      GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
541      TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
601      TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
661      GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
721      AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
781      CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
841      CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
901      AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
961      CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
1021     CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
1081     CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
1141     CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
1201     ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
1261     TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
1321     TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
1381     GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
1441     CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
1501     AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
1561     AGCGTCCTGT TCTGA

Quality Control:cDNA sequencing results is available upon request.