ALPI / IAP Protein (His Tag)

Alkaline phosphatase, intestinal Protein Datasheet

ALPI / IAP Protein Product Information

• Synonym: IAP
• Protein Construction: A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1- Asp 503) ,without the pro peptide, was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

ALPI / IAP Protein QC Testing

• Purity: >95 % as determined by SDS-PAGE.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).
  The specific activity is > 1,000 pmoles/min/μg.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 20
• Molecular Mass: The secreted recombinant human ALPI consists of 495 amino acids and has a predicted molecular mass of 54 kDa. The apparent molecular mass of rhALPI is approximately 66 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ALPI / IAP protein

ALPI / IAP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ALPI / IAP Protein Description

Alkaline phosphatase, intestinal also known as ALPI is digestive brush-border enzyme which is upregulated during small intestinal epithelial cell differentiation. ALPI is also referred to as IAP and it is released into the lumen after a high-fat diet. ALPI / IAP is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. Lysophosphatidylcholine (LysoPC) releases IAP into the apical medium more than other intestinal brush-border enzymes, sucrose, aminopeptidase N, and lactase, without comparable lactate dehydrogenase release or cell injury. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver / bone / kidney (tissue non-specific). Intestinal ALP shows 86.5% amino acid identity to placental ALP and 56.6% amino acid identity to liver / bone / kidney ALP.

References

1. Nakano T. et al., 2009, Am J Physiol Gastrointest Liver Physiol. 297 (1): 207-14.
2. Henthorn PS. et al., 1987, Proc Natl Acad Sci. 84 (5): 1234-8.
3. Oetting WS. et al., 2003, Pigment Cell Res. 16 (3): 307-11.