Human ALOX5AP Gene cDNA Clone / ORF Clone

ALOX5AP cDNA Clone Datasheet

ALOX5AP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001629.2
• cDNA Size: 486
• Gene Synonym: FLAP; ALOX5AP
• Source: Human

ALOX5AP cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens arachidonate 5-lipoxygenase-activating protein DNA.
• Vector: pMD18-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

ALOX5AP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ALOX5AP cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

ALOX5AP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATCAAG AAACTGTAGG CAATGTTGTC CTGTTGGCCA TCGTCACCCT CATCAGCGTG
61       GTCCAGAATG GATTCTTTGC CCATAAAGTG GAGCACGAAA GCAGGACCCA GAATGGGAGG
121      AGCTTCCAGA GGACCGGAAC ACTTGCCTTT GAGCGGGTCT ACACTGCCAA CCAGAACTGT
181      GTAGATGCGT ACCCCACTTT CCTCGCTGTG CTCTGGTCTG CGGGGCTACT TTGCAGCCAA
241      GTTCCTGCTG CGTTTGCTGG ACTGATGTAC TTGTTTGTGA GGCAAAAGTA CTTTGTCGGT
301      TACCTAGGAG AGAGAACGCA GAGCACCCCT GGCTACATAT TTGGGAAACG CATCATACTC
361      TTCCTGTTCC TCATGTCCGT TGCTGGCATA TTCAACTATT ACCTCATCTT CTTTTTCGGA
421      AGTGACTTTG AAAACTACAT AAAGACGATC TCCACCACCA TCTCCCCTCT ACTTCTCATT
481      CCCTAA

 

Quality Control:cDNA sequencing results is available upon request.