ALOX5AP / FLAP Protein (His Tag)

Arachidonate 5-lipoxygenase-Activating Protein

ALOX5AP / FLAP Protein Product Information

• Synonym: FLAP, ALOX5AP
• Protein Construction: A DNA sequence encoding the full length of human ALOX5AP (NP_001620.2) (Met 1- Pro 161) was fused with a polyhistidine tag at the carboxy-terminus
• Source: Human
• Expression Host: Baculovirus-Insect cells

ALOX5AP / FLAP Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human ALOX5AP consists of 167 amino acids and predicts a molecular mass of 19 KDa as estimated in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris,150mM NaCl, 10%gly, pH 7.4, 0.5%triton , 5mM DTT
• SDS-PAGE: ALOX5AP protein

ALOX5AP / FLAP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ALOX5AP / FLAP Protein Description

Arachidonate 5-lipoxygenase-activating protein, also known as FLAP, MK-886-binding protein and ALOX5AP, is a multi-pass membrane protein which belongs to the MAPEG family. ALOX5AP is required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). ALOX5AP anchors ALOX5 to the membrane. It binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. ALOX5AP also binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. A key role in the inflammatory pathway is played by the ALOX5AP, which is known to influence the risk of coronary heart disease (CHD) in hypercholesterolemia (FH). Variants in the ALOX5AP gene are associated with the presence of xanthomas in FH patients. Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke, also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function.

References

1. Dixon R.A.F., et al., 1990, Nature. 343: 282-284.
2. Mancini J.A., et al., 1993, FEBS Lett. 318:277-281.
3. Kostulas, K. et al., 2007, J Neurol Sci. 263 (1-2): 113-7.
4. Oosterveer, DM. et al., 2009,Atherosclerosis. 206 (1): 223-7.