ALK-1 Protein, Cynomolgus, Recombinant (Fc Tag)

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ALK-1 Protein, Cynomolgus, Recombinant (Fc Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3-E1 cells.
The ED50 for this effect is typically 1-5 ng/ml.
Protein Construction
A DNA sequence encoding the cynomolgus ACVRL1 (Met1-Gln118) was expressed with the Fc region of human IgG1 at the C-terminus.
Expressed Host
HEK293 Cells
Species
Cynomolgus
Predicted N Terminal
Asp 22
Molecule Mass
The recombinant cynomolgus ACVRL1 is a disulfide-linked homodimer. The reduced monomer comprises 338 amino acids and has a calculated molecular mass of 37.8 KDa.The apparent molecular mass of the protein is approximately 53 KDa respectively in SDS-PAGE.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ALK-1 Protein, Cynomolgus, Recombinant (Fc Tag): Images

Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 1-5 ng/ml.

ALK-1 Background Information

Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.
Full Name
activin A receptor type II-like 1
References
  • French Rendu-Osler network,et al. (2004) Molecular screening of ALK1/ACVRL1 and ENG genes in hereditary hemorrhagic telangiectasia in France. Hum Mutat. 23(4): 289-299.
  • Simon M, et al. (2006) Association of a polymorphism of the ACVRL1 gene with sporadic arteriovenous malformations of the central nervous system. J Neurosurg. 104(6): 945-9.
  • Argyriou L, et al. (2006) Novel mutations in the ENG and ACVRL1 genes causing hereditary hemorrhagic teleangiectasia. Int J Mol Med. 17(4):655-9.
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