ALDOB / Aldolase B Protein (GST Tag)

Aldolase B, fructose-bisphosphate Protein Datasheet

ALDOB / Aldolase B Protein Product Information

• Synonym: RP11-490D19.1, ALDB, ALDO2
• Protein Construction: A DNA sequence encoding the human ALDOB (P05062) (Ala 2-Tyr 364) was fused with the GST tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

ALDOB / Aldolase B Protein QC Testing

• Purity: >88% as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human ALDOB/GST chimera consists of 597 amino acids and has a predicted molecular mass of 66.5 kDa. It migrates as an approxiamtely 60 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ALDOB / Aldolase B protein

ALDOB / Aldolase B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ALDOB / Aldolase B Protein Description

Aldolase B (ALDOB), also known as fructose-bisphosphate aldolase B or liver-type aldolase, is an important enzyme classified into isoenzymes (A, B, and C) of the class Ⅰfructose 1,6-bisphosphate aldolase enzyme, and plays a key role in both glycolysis and gluconeogenesis. Aldolase B is composed of four subunits. Each subunit has a molecular weight of about 36kDa and contains an eight-stranded α/β barrel, which encloses lysine 229. Aldolase B plays a key role in carbohydrate metabolism as it catalyzes one of the major steps of the glycolytic-gluconeogenic pathway. Genetic mutatons leading to defects in aldolase B results in a condition called hereditary fructose intolerance. This homotetrameric enzyme contribute to catalyze the reversible reaction of fructose 1,6-bisphosphate(FBP) into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate (DHAP) as well as the reversible cleavage of fructose 1-phosphate (F1P) into glyceraldehydes and dihydroxyacetone phosphate.

References

1. Cox TM. 1994, FASEB J. 8(1): 62-71.
2. Malay AD. et al., 2005, J Mol Biol. 347 (1): 135-44.
3. Susan PP. et al., 2001, J Cell Physiol. 187 (1): 48-58.