Human ALDH7A1 Gene cDNA Clone / ORF Clone

ALDH7A1 cDNA Clone Datasheet

ALDH7A1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001182.3
• cDNA Size: 1536
• Gene Synonym: EPD; PDE; ATQ1; FLJ11738; FLJ92814; ALDH7A1
• Source: Human

ALDH7A1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens aldehyde dehydrogenase 7 family, member A1 DNA.
• Vector: Cloning Vector: pMD18-T Simple Vector.

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1231 A/C resulting in the amino acid Lys substitution by Gln.

ALDH7A1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ALDH7A1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

ALDH7A1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCCACTC TCCTCATCAA TCAGCCCCAG TATGCGTGGC TGAAAGAGCT GGGGCTCCGC
61       GAGGAAAACG AGGGCGTGTA TAATGGAAGC TGGGGAGGCC GGGGAGAGGT TATTACGACC
121      TATTGCCCTG CTAACAACGA GCCAATAGCA AGAGTCCGAC AGGCCAGTGT GGCAGACTAT
181      GAAGAAACTG TAAAGAAAGC AAGAGAAGCA TGGAAAATCT GGGCAGATAT TCCTGCTCCA
241      AAACGAGGAG AAATAGTAAG ACAGATTGGC GATGCCTTGC GGGAGAAGAT CCAAGTACTA
301      GGAAGCTTGG TGTCTTTGGA GATGGGGAAA ATCTTAGTGG AAGGTGTGGG TGAAGTTCAG
361      GAGTATGTGG ATATCTGTGA CTATGCTGTT GGTTTATCAA GGATGATTGG AGGACCTATC
421      TTGCCTTCTG AAAGATCTGG CCATGCACTG ATTGAGCAGT GGAATCCCGT AGGCCTGGTT
481      GGAATCATCA CGGCATTCAA TTTCCCTGTG GCAGTGTATG GTTGGAACAA CGCCATCGCC
541      ATGATCTGTG GAAATGTCTG CCTCTGGAAA GGAGCTCCAA CCACTTCCCT CATTAGTGTG
601      GCTGTCACAA AGATAATAGC CAAGGTTCTG GAGGACAACA AGCTGCCTGG TGCAATTTGT
661      TCCTTGACTT GTGGTGGAGC AGATATTGGC ACAGCAATGG CCAAAGATGA ACGAGTGAAC
721      CTGCTGTCCT TCACTGGGAG CACTCAGGTG GGAAAACAGG TGGGCCTGAT GGTGCAGGAG
781      AGGTTTGGGA GAAGTCTGTT GGAACTTGGA GGAAACAATG CCATTATTGC CTTTGAAGAT
841      GCAGACCTCA GCTTAGTTGT TCCATCAGCT CTCTTCGCTG CTGTGGGAAC AGCTGGCCAG
901      AGGTGTACCA CTGCGAGGCG ACTGTTTATA CATGAAAGCA TCCATGATGA GGTTGTAAAC
961      AGACTTAAAA AGGCCTATGC ACAGATCCGA GTTGGGAACC CATGGGACCC TAATGTTCTC
1021     TATGGGCCAC TCCACACCAA GCAGGCAGTG AGCATGTTTC TTGGAGCAGT GGAAGAAGCA
1081     AAGAAAGAAG GTGGCACAGT GGTCTATGGG GGCAAGGTTA TGGATCGCCC TGGAAATTAT
1141     GTAGAACCGA CAATTGTGAC AGGTCTTGGC CACGATGCGT CCATTGCACA CACAGAGACT
1201     TTTGCTCCGA TTCTCTATGT CTTTAAATTC CAGAATGAAG AAGAGGTCTT TGCATGGAAT
1261     AATGAAGTAA AACAGGGACT TTCAAGTAGC ATCTTTACCA AAGATCTGGG CAGAATCTTT
1321     CGCTGGCTTG GACCTAAAGG ATCAGACTGT GGCATTGTAA ATGTCAACAT TCCAACAAGT
1381     GGGGCTGAGA TTGGAGGTGC CTTTGGAGGA GAAAAGCACA CTGGTGGTGG CAGGGAGTCT
1441     GGCAGTGATG CCTGGAAACA GTACATGAGA AGGTCTACTT GTACTATCAA CTACAGTAAA
1501     GACCTTCCTC TGGCCCAAGG AATCAAGTTT CAGTAA

 

Quality Control:cDNA sequencing results is available upon request.