Anti-ALDH7A1 Antibody

Cat: 11614-RP02
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All ALDH7A1 Reagents
CRO Services
Product recommended

All ALDH7A1 Reagents
Anti-ALDH7A1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-ALDH7A1 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human ALDH7A1
Immunogen
Recombinant Human ALDH7A1 protein (Catalog#11614-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human ALDH7A1 (rh ALDH7A1; Catalog#11614-H07E; P49419-2; Ser 2-Gln 511). ALDH7A1 specific IgG was purified by human ALDH7A1 affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ALDH7A1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:5000-1:25000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ALDH7A1 Antibody (Rabbit Polyclonal antibody) Images
Human ALDH7A1 Immunohistochemistry(IHC) 4719
Immunochemical staining of human ALDH7A1 in human liver (from 2 donors) with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections. The image showing positive staining of hepatocyte.
Human ALDH7A1 Immunohistochemistry(IHC) 4721
Immunochemical staining of human ALDH7A1 in human hepatoma (from 3 donors) with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections.
Human ALDH7A1 Immunoprecipitation(IP) 8098

ALDH7A1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-ALDH7A1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ALDH7A1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

Observed band size: 55 kDa

Human ALDH7A1 Western blot (WB) 13425

Anti-ALDH7A1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: SH-SY5Y Whole Cell Lysate

Lane C: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:58 kDa

Observed band size:58 kDa

(We are unsure as to the identity of these extra bands.)

Anti-ALDH7A1 Antibody Alternative Names
Anti-ATQ1 Antibody;Anti-EPD Antibody;Anti-PDE Antibody
ALDH7A1 Background Information

ALDH7A1 (Aldehyde dehydrogenase 7 family, member A1) is a member of subfamily 7 in the aldehyde dehydrogenase family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. Mammalian ALDH7A1 is homologous to plant ALDH7B1 which protects against various forms of stress such as increased salinity, dehydration and treatment with oxidants or pesticides. In mammals, ALDH7A1 is known to play a primary role during lysine catabolism through the NAD+-dependent oxidative conversion of aminoadipate semialdehyde (AASA) to its corresponding carboxylic acid, α-aminoadipic acid. Deleterious mutations in human ALDH7A1 are responsible for pyridoxine-dependent and folinic acid-responsive seizures. ALDH7A1 is a novel aldehyde dehydrogenase expressed in multiple subcellular compartments that protects against hyperosmotic stress by generating osmolytes and metabolizing toxic aldehydes.

Full Name
aldehyde dehydrogenase 7 family, member A1
References
  • Brocker C, et al. (2011) Aldehyde dehydrogenase 7A1 (ALDH7A1) attenuates reactive aldehyde and oxidative stress induced cytotoxicity. Chem Biol Interact. 191(1-3): 269-77.
  • Brocker C, et al. (2010) Aldehyde dehydrogenase 7A1 (ALDH7A1) is a novel enzyme involved in cellular defense against hyperosmotic stress. J Biol Chem. 285(24): 18452-63.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ALDH7A1 Antibody Rabbit Monoclonal WB,ELISA,IP 11614-R105
    Anti-ALDH7A1 Antibody Mouse Monoclonal IHC-P 11614-MM11
    Anti-ALDH7A1 Antibody Rabbit Polyclonal ELISA 11614-RP01
    Anti-ALDH7A1 Antibody Mouse Monoclonal ELISA 11614-MM02
    Anti-ALDH7A1 Antibody Mouse Monoclonal WB 11614-MM04
    Anti-ALDH7A1 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 11614-R101
    Anti-ALDH7A1 Antibody (FITC) Rabbit Monoclonal FCM 11614-R101-F
    Anti-ALDH7A1 Antibody (PE) Rabbit Monoclonal FCM 11614-R101-P
    info info
    添加购物车成功! 添加购物车失败!