ALDH7A1 Antibody

ALDH7A1 Antibody Datasheet

ALDH7A1 Antibody Product Information

• Immunogen: Recombinant Human ALDH7A1 protein (Catalog#11614-H07E)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 04
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ALDH7A1 (rh ALDH7A1; Catalog#11614-H07E; P49419-2; Ser 2-Gln 511). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

ALDH7A1 Antibody Usage Guide

• Specificity: Human ALDH7A1
• Western blot: This antibody can be used at 2-5 μg/mL with the appropriate secondary reagents to detect Human ALDH7A1 in WB.

  A Sample (whole cell lysate) Hela Sample Volume (cells/lane) 2.5×105 Gel 13% SDS-PAGE reducing gel Recommended Concentration 2-5 μg/ml Secondary Antibody Dylight 800-Labeled Antibody To Mouse IgG (H+L), at 1:20000 dilution.

• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ALDH7A1 Antibody Background

Aldehyde dehydrogenase 7 family member A1 also known as ALDH7A1 and antiquitin, is an enzyme which is a member of subfamily 7 in the aldehyde dehydrogenase family. The aldehyde dehydrogenase (ALDH) superfamily represents a divergently related group of enzymes that metabolize a wide variety of endogenous and exogenous aldehydes. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. ALDH7A1 functions as an aldehyde dehydrogenase in the pipecolic acid pathway of lysine catabolism. Mutations in ALDH7A1 cause pyridoxine-dependent epilepsy, which involves a combination of various seizure types and is responsive to immediate administration of pyridoxine hydrochloride.

References

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