Human ALDH3A1 Gene cDNA Clone / ORF Clone

Human ALDH3A1 cDNA Clone Datasheet

ALDH3A1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001135168.1
• cDNA Size: 1362
• Gene Synonym: ALDH3; ALDHIII; MGC10406; ALDH3A1
• Source: Human

ALDH3A1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens aldehyde dehydrogenase 3 family, member A1 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 400 T/G and 985 C/G, resulting in the amino acid ser substitution by ala,Pro substitution by ala.

ALDH3A1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ALDH3A1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ALDH3A1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCAAGA TCAGCGAGGC CGTGAAGCGC GCCCGCGCCG CCTTCAGCTC GGGCAGGACC
61       CGTCCGCTGC AGTTCCGGAT CCAGCAGCTG GAGGCGCTGC AGCGCCTGAT CCAGGAGCAG
121      GAGCAGGAGC TGGTGGGCGC GCTGGCCGCA GACCTGCACA AGAATGAATG GAACGCCTAC
181      TATGAGGAGG TGGTGTACGT CCTAGAGGAG ATCGAGTACA TGATCCAGAA GCTCCCTGAG
241      TGGGCCGCGG ATGAGCCCGT GGAGAAGACG CCCCAGACTC AGCAGGACGA GCTCTACATC
301      CACTCGGAGC CACTGGGCGT GGTCCTCGTC ATTGGCACCT GGAACTACCC CTTCAACCTC
361      ACCATCCAGC CCATGGTGGG CGCCATCGCT GCAGGGAACG CAGTGGTCCT CAAGCCCTCG
421      GAGCTGAGTG AGAACATGGC GAGCCTGCTG GCTACCATCA TCCCCCAGTA CCTGGACAAG
481      GATCTGTACC CAGTAATCAA TGGGGGTGTC CCTGAGACCA CGGAGCTGCT CAAGGAGAGG
541      TTCGACCATA TCCTGTACAC GGGCAGCACG GGGGTGGGGA AGATCATCAT GACGGCTGCT
601      GCCAAGCACC TGACCCCTGT CACGCTGGAG CTGGGAGGGA AGAGTCCCTG CTACGTGGAC
661      AAGAACTGTG ACCTGGACGT GGCCTGCCGA CGCATCGCCT GGGGGAAATT CATGAACAGT
721      GGCCAGACCT GCGTGGCCCC TGACTACATC CTCTGTGACC CCTCGATCCA GAACCAAATT
781      GTGGAGAAGC TCAAGAAGTC ACTGAAAGAG TTCTACGGGG AAGATGCTAA GAAATCCCGG
841      GACTATGGAA GAATCATTAG TGCCCGGCAC TTCCAGAGGG TGATGGGCCT GATTGAGGGC
901      CAGAAGGTGG CTTATGGGGG CACCGGGGAT GCCGCCACTC GCTACATAGC CCCCACCATC
961      CTCACGGACG TGGACCCCCA GTCCGCGGTG ATGCAAGAGG AGATCTTCGG GCCTGTGCTG
1021     CCCATCGTGT GCGTGCGCAG CCTGGAGGAG GCCATCCAGT TCATCAACCA GCGTGAGAAG
1081     CCCCTGGCCC TCTACATGTT CTCCAGCAAC GACAAGGTGA TTAAGAAGAT GATTGCAGAG
1141     ACATCCAGTG GTGGGGTGGC GGCCAACGAT GTCATCGTCC ACATCACCTT GCACTCTCTG
1201     CCCTTCGGGG GCGTGGGGAA CAGCGGCATG GGATCCTACC ATGGCAAGAA GAGCTTCGAG
1261     ACTTTCTCTC ACCGCCGCTC TTGCCTGGTG AGGCCTCTGA TGAATGATGA AGGCCTGAAG
1321     GTCAGATACC CCCCGAGCCC GGCCAAGATG ACCCAGCACT GA

 

Quality Control:cDNA sequencing results is available upon request.