Rat ALCAM Gene cDNA Clone / ORF Clone

ALCAM cDNA Clone Datasheet

ALCAM cDNA Clone Product Information

• Gene Bank Ref.ID: NM_031753.1
• cDNA Size: 1752
• Gene Synonym: Alcam
• Source: Rat

ALCAM cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus activated leukocyte cell adhesion molecule DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

ALCAM cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ALCAM cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ALCAM cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCATCTA AAGGGTCCCC TTCTTGCCGT CTGGTTTTCT GCCTCTTGAT CTCCGCCGCG
61       GTCCTCAGAC CAGGCCTCGG ATGGTACACT GTCAACTCAG CATACGGAGA TACCATTGTC
121      ATGCCTTGCA GACTGGATGT ACCTCAGAAC CTCATGTTTG GCAAATGGAA ATATGAAAAG
181      CCTGATGGGT CCCCAGTATT TATTGCATTC AGATCTTCTA CAAAGAAAAG TGTGCAGTAT
241      GATGATGTAC CAGAGTACAA GGACAGACTG AGCCTCTCAG AAAACTACAC TCTGTCTATC
301      AACAATGCAA AGATCAGTGA CGAAAAGAGA TTTGTGTGCA TGCTAGTGAC CGAGGACAAC
361      GTGTTTGAGG CACCTACCCT GGTCAAGGTG TTCAAGCAAC CATCTAAACC TGAAATTGTA
421      AACAGAGCAG CGTTTCTTGA AACAGAGCAG CTAAAAAAGT TAGGTGACTG CATTTCAAGA
481      GACAGTTACC CAGACGGCAA CATCACGTGG TATAGGAATG GGAAAGTGCT ACAGCCTGTT
541      GATGGAGAGG TGTCCATACT TTTTAAAAAG GAAATTGATC CAGGTACTCA GTTGTATACC
601      ATGACTTCCT CCTTGGAGTA CAAGACAACC AAGTCTGACA TACAAATGCC ATTCACCTGT
661      TCTGTGACAT ATTATGGACC TTCGGGCCAG AAAACAATTT ATTCTGAACA AGCAATCTTT
721      GATATTTACT ATCCTACAGA GCAGGTGACA ATACAAGTAC TGCCACCAAA AAATGCCATC
781      AAAGAAGGGG ACAACATCAC CCTTCAGTGC TTGGGGAATG GCAACCCACC TCCTGAGGAG
841      TTCATGTTTT ACTTACCAGG GCAGGCTGAA GGCATAAGAA GCTCAAACAC TTACACACTG
901      ACAGACGTGA GACGCAATGC CACCGGGGAC TACAAATGTT CTCTCATCGA CCAAAGAAAC
961      ATGGCAGCTT CAACAACCAT CACTGTTCAC TACTTGGATT TATCCTTAAA CCCAAGTGGG
1021     GAAGTGACCA AGCAGATCGG TGATACCCTG CCTGTGTCTT GCACAATATC TGCAAGTAGG
1081     AATGCAACTG TGGTGTGGAT GAAGGATAAC ATCAGGCTCC GATCTAGTCC ATCCTTTTCT
1141     AGTCTTCATT ATCAGGATGC TGGGAACTAT GTCTGTGAAA CTGCTCTTCA GGAGGTTGAG
1201     GGACTGAAGA AAAGGGAGTC GCTGACCCTC ATCGTAGAAG GAAAACCTCA AATCAAAATG
1261     ACAAAGAAAA CTGATCCCAG TGGACTGTCT AAGACTATAA TCTGCCATGT GGAAGGGTTT
1321     CCAAAGCCAG CTATACAGTG GACCATTACC GGCAGTGGAA GCGTCATAAA CCAAACAGAG
1381     GAGTCTCCTT ATATTAATGG CAGGTATTAT AGTAAAATTA TCATTTCCCC TGAGGAGAAT
1441     GTTACATTAA CTTGCACAGC AGAAAACCAA CTGGAGAGAA CAGTAAACTC CCTGAATGTC
1501     TCTGCGATAA GTATTCCAGA ACACGATGAG GCAGACGATA TAAGTGATGA AAACAGAGAA
1561     AAGGTGAATG ACCAGGCCAA ACTAATTGTG GGCATTGTGG TTGGTCTCCT CCTCGCCGCC
1621     CTCGTCGCCG GTGTCGTCTA CTGGCTGTAC ATGAAGAAAT CGAAAACTGC ATCAAAACAT
1681     GTAAACAAGG ACCTTGGTAA TATGGAGGAG AATAAAAAGC TAGAAGAAAA CAATCACAAA
1741     ACAGAAGCCT AA

 

Quality Control:cDNA sequencing results is available upon request.