Rat ALCAM / CD166 Protein (His Tag)

Activated leukocyte cell adhesion molecule Protein Datasheet

ALCAM / CD166 Protein Product Information

• Synonym: ALCAM
• Protein Construction: A DNA sequence encoding the rat ALCAM(O35112)(Met1-Lys527) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

ALCAM / CD166 Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Trp 28
• Molecular Mass: The recombinant rat ALCAM comprises 511 amino acids and predicts a molecular mass of 57.5 kDa. The apparent molecular mass of the recombinant protein is approximately 68-91 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ALCAM / CD166 protein

ALCAM / CD166 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ALCAM / CD166 Protein Description

ALCAM (activated leukocyte cell adhesion molecule) is a type I  membrane glycoprotein and a member of the immunoglobulin superfamily. It is also known as CD166, MEMD, SC-1/DM-GRASP in the chicken, and KG-CAM in the rat. ALCAM / CD166 has five extracellular immunoglobulin-like domains which facilitates heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. While expressed in a wide variety of tissues and cells, ALCAM is restricted to subsets of cells usually involved in dynamic growth and / or migration processes. ALCAM is highly associated not only with embryonic hematopoiesis but also vasculoangiogenesis. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other unidentified brain proteins, and thus is Involved in neurite extension by neurons via heterophilic and homophilic manners. ALCAM / CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- cells and B-cells to activated leukocytes. Further, ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines, suggesting a role in tumor migration.

References

1. Bowen, M. A. et al., 1995, J. Exp. Med. 181:2213-2220.
2. Aruffo, A. et al., 1997, Immunol. Today. 18: 498-504.
3. Degen, W. G. et al., 1998, Am. J. Pathol. 152: 805-813.
4. Van, K. L. et al., 2001, J. Biol. Chem. 276: 25783-257890.
5. Hirata, H. et al., 2006, Cells Tissues Organs. 184 (3-4):172-180.
6. Jezierska, A. et al., 2006, Med Sci Monit.12 (7): BR245-256.