Mouse AKT3 Gene cDNA Clone / ORF Clone

AKT3 cDNA Clone Datasheet

AKT3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011785.3
• cDNA Size: 1440
• Gene Synonym: AI851531; D930002M15Rik; Akt3
• Source: Mouse

AKT3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus thymoma viral proto-oncogene 3 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

AKT3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

AKT3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

AKT3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCGATG TTACCATTGT GAAAGAAGGT TGGGTTCAGA AGAGGGGAGA ATATATAAAA
61       AACTGGAGGC CAAGATACTT CCTTTTGAAG ACAGATGGCT CATTCATAGG CTATAAGGAG
121      AAACCTCAAG ATGTGGACTT ACCTTATCCC CTCAACAACT TCTCAGTGGC AAAATGTCAG
181      TTAATGAAAA CAGAACGACC AAAGCCAAAT ACATTTATTA TCAGATGTCT TCAGTGGACC
241      ACTGTTATAG AGAGAACATT TCATGTAGAT ACACCAGAGG AAAGAGAAGA GTGGACGGAA
301      GCTATCCAAG CCGTAGCCGA CCGATTGCAG AGGCAAGAGG AGGAGAGGAT GAATTGTAGC
361      CCAACCTCAC AGATTGATAA TATAGGAGAA GAAGAGATGG ATGCGTCTAC AACCCATCAT
421      AAAAGAAAGA CGATGAATGA TTTTGACTAT TTGAAACTAC TAGGTAAAGG CACTTTTGGG
481      AAAGTTATTT TGGTTCGAGA GAAGGCAAGT GGAAAATACT ATGCTATGAA GATTCTGAAG
541      AAAGAAGTCA TTATTGCAAA GGATGAAGTG GCACACACTC TTACTGAAAG CAGAGTACTA
601      AAGAACACCA GACATCCATT TTTAACATCC TTGAAATATT CCTTCCAGAC AAAAGACCGT
661      TTGTGTTTTG TGATGGAATA TGTTAATGGC GGAGAGCTGT TTTTCCATTT GTCGAGAGAG
721      CGAGTGTTCT CTGAGGACCG CACACGTTTC TATGGTGCAG AAATTGTCTC TGCTTTGGAC
781      TATCTACATT CTGGAAAGAT TGTGTACCGT GATCTCAAGT TGGAGAATTT GATGCTAGAT
841      AAGGATGGCC ATATAAAAAT TACGGATTTT GGGCTTTGCA AAGAAGGGAT CACAGATGCA
901      GCTACCATGA AGACATTCTG TGGCACACCA GAGTACCTGG CACCAGAGGT ATTAGAAGAT
961      AATGACTATG GCCGAGCCGT GGACTGGTGG GGCTTAGGTG TTGTCATGTA TGAAATGATG
1021     TGTGGAAGGT TGCCTTTCTA CAACCAGGAT CATGAGAAAC TCTTTGAATT AATACTAATG
1081     GAAGACATTA AATTCCCCCG AACACTCTCT TCAGATGCAA AATCATTGCT TTCAGGGCTC
1141     TTGATAAAGG ATCCAAATAA ACGCCTTGGT GGAGGGCCAG ATGATGCAAA AGAAATCATG
1201     AGGCATAGTT TTTTTTCTGG AGTAAACTGG CAAGATGTAT ATGACAAAAA GCTTGTACCT
1261     CCTTTTAAGC CTCAAGTAAC ATCTGAAACA GACACCCGAT ATTTTGATGA AGAATTTACA
1321     GCTCAGACTA TTACAATAAC ACCACCTGAA AAGTATGACG ACGACGGCAT GGACGGCATG
1381     GACAACGAGC GGCGGCCACA CTTCCCTCAG TTCTCCTACT CTGCAAGCGG ACGGGAATAA

 

Quality Control:cDNA sequencing results is available upon request.