All AKT3 reagents are produced in house and quality controlled, including 1 AKT3 Antibody, 28 AKT3 Gene, 3 AKT3 Lysate, 3 AKT3 Protein, 2 AKT3 qPCR. All AKT3 reagents are ready to use.
Recombinant AKT3 proteins are expressed by Baculovirus-Insect Cells with fusion tags as N-GST & His, N-cleavage, N-GST.
AKT3antibodies are validated with different applications, which are ELISA.
AKT3cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each AKT3 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Expression host: Baculovirus-Insect Cells
v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.